Evaluating the use of 3-(p-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions.docVIP

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Evaluating the use of 3-(p-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions.doc

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Evaluating the use of 3-(p-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions

Geochemical Transactions BioMedCentral Methodology Open Access Evaluating the use of 3-(p-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions Corey A Cohn*1,2,5, Christopher E Pedigo3, Shavonne N Hylton2, Sanford R Simon4 and Martin AA Schoonen1,2 Address: 1Center for Environmental Molecular Science, Stony Brook University, Stony Brook, NY 11794-2100, USA, 2Department of Geosciences, Stony Brook University, Stony Brook, NY 11794-2100, USA, 3Department of Biological Sciences, Stony Brook University, Stony Brook, NY 11794, USA, 4Department of Pathology, Stony Brook University Hospital, Stony Brook, NY 11794, USA and 5National Research Centre for the Working Environment, Lerso Parkalle 105, 2100 Copenhagen, Denmark Email: Corey A Cohn* - coreycohn@; Christopher E Pedigo - christopherpedigo@; Shavonne N Hylton - snhylton@; Sanford R Simon - ssimon@; Martin AA Schoonen - mschoonen@ * Corresponding author Published: 11 August 2009 Received: 5 January 2009 Accepted: 11 August 2009 Geochemical Transactions 2009, 10:8 doi:10.1186/1467-4866-10-8 This article is available from: /content/10/1/8 ? 2009 Cohn et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Given the importance of highly reactive oxygen species (hROS) as reactants in a wide range of biological, photochemical, and environmental systems there is an interest in detection and quantification of these species. The extreme reactivity of the hROS, which includes hydroxyl radicals, presents an analytical challenge. 3-(p-Aminophenyl) fluorescein (APF) is a relatively new probe used for measuring hROS. Here, we further evaluate the use of APF as a metho