Evaluation of the Immunity Activity of Glycyrrhizin in AR Mice.docVIP

Molecules 2012, 17, 716-727; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Evaluation of the Immunity Activity of Glycyrrhizin in AR Mice Xiao-Lan Li 1,* and Ai-Guo Zhou 2 1 Ear-Nose-Throat (ENT) Department of the 1st Hospital Associated to the ShiHeZi University, ShiHeZi City 832008, XinJiang, China 2 Acute Surgery Department of the 1st Hospital Associated to the ShiHeZi University, ShiHeZi City 832008, XinJiang, China; E-Mail: zhouagsu207@ * Author to whom correspondence should be addressed; E-Mail: lixlsdoc158@; Tel.: +86-0993-285-8573; Fax: +86-0993-282-5993. Received: 12 December 2011; in revised form: 29 December 2011 / Accepted: 29 December 2011 / Published: 12 January 2012 Abstract: In this study, we evaluated effect of glycyrrhizin on immunity function in allergic rhinitis (AR) mice. The AR mice model were induced by dripping ovalbumin in physiological saline (2 mg mL?1, 10 μL) into the bilateral nasal cavities using a micropipette. After the AR model was induced, mice were randomly divided into six groups: the normal control, model, lycopene 20 mg kg?1 (as positive control drug) group, and glycyrrhizin 10, 20, 30 mg kg?1 groups. After the sensitization day 14, lycopene (20 mg/kg BW) and glycyrrhizin (10, 20 and 30 mg/kg BW) were given orally for 20 days once a day. Mice in the normal control and model groups were given saline orally once a day for 20 days. Results showed that glycyrrhizin treatment could dose-dependently significantly reduce blood immunoglobulin E (IgE), interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-6 (IL-6), nitrous oxide (NO), tumor necrosis factor-alpha (TNF-α) levels and nitrous oxide synthase (NOS) activity and enhance blood immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM), interleukin-2 (IL-2) and interleukin-12 (IL-12) levels in AR mice. Furthermore, glycyrrhizin treatment