Evaluation on Anti-hepatitis Viral Activity of Vitis vinifer L.docVIP

Molecules 2010, 15, 7415-7422; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Evaluation on Anti-hepatitis Viral Activity of Vitis vinifer L Tao Liu 1, Jun Zhao 2, Haibo Li 3 and Long Ma 1,* 1 College of Public Health, Xinjiang Medical University, Urumqi, 830011, China 2 Xinjiang Key Laboratory for Research and Development of Uighur Medicine, Institute of Materia Medica of Xinjiang, Urumqi, 830002, China; E-Mail: zhaojun21cn@163.com (J.Z.) 3 Nantong Center for Disease Control and Prevention, Nantong, 226007, China; E-Mail: lihaibo1972@163.com (H.L.) * Author to whom correspondence should be addressed; E-Mail: xjmult @ 163.com; Tel.: +86-991-4365317. Received: 20 August 2010; in revised form: 15 October 2010 / Accepted: 19 October 2010 / Published: 22 October 2010 Abstract: Suosuo grape (Vitis vinifer L) is traditionally used as a therapeutic agent for measles and hepatitis by the ethnic Uighurs. This work aimed to investigate the anti-HBV effect of total triterpene (VTT), total flavonoids (VTF) and total polysaccharides (VTP) from Suosuo grape, and their synergistic effects were also tested. The viral antigens of cellular secretion, HBsAg and HBeAg, were determined by enzyme linked immunosorbent assay (ELISA).The quantity of HBV-DNA released in the supernatant was assayed by real- time PCR. It was found that it effectively suppressed the secretion of HBsAg and HBeAg from HepG2.2.15 cells in a dose-dependent manner, as well as the HBV DNA. The results of orthogonal design experiment showed that the combination of VTT 20 μg/mL, VTF 50 μg/mL and VTP 50 μg/mL had the best optimistic inhibitory effects on HBeAg secretion. Keywords: Vitis vinifer L; anti-hepatitis viral activity; orthogonal design  Molecules 2010, 15 7416 1. Introduction Hepatitis B virus (HBV) infections remain a major public health problem for nearly 350 million people. HBV