Expression of EBV Encoded viral RNA 1, 2 and anti-inflammatory Cytokine (interleukin-10) in FFPE lymphoma specimens a preliminary study for diagnostic implication in Pakistan.docVIP

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Expression of EBV Encoded viral RNA 1, 2 and anti-inflammatory Cytokine (interleukin-10) in FFPE lymphoma specimens a preliminary study for diagnostic implication in Pakistan.doc

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Expression of EBV Encoded viral RNA 1, 2 and anti-inflammatory Cytokine (interleukin-10) in FFPE lymphoma specimens a preliminary study for diagnostic implication in Pakistan

SheikhandQadriDiagnosticPathology2011,6:70 /content/6/1/70 METHODOLOGY OpenAccess ExpressionofEBVEncodedviralRNA1,2and anti-inflammatoryCytokine(interleukin-10)in FFPElymphomaspecimens:apreliminarystudy fordiagnosticimplicationinPakistan TaimoorISheikh*?andIshtiaqQadri? Abstract Background:EpsteinBarrVirus(EBV)playsasignificantroleasacofactorintheprocessoftumorigenesisandhas consistentlybeenassociatedwithavarietyofmalignancies.EBVencodedRNAs(EBER1andEBER2)arethemost abundantviraltranscriptsinlatentlyEBV-infectedcellsandtheirroleinviralinfectionisstillunclear.FormalinFixed ParaffinEmbedded(FFPE)tissuesofsurgicallyremovedcarcinomabiopsiesarewidelyavailableformbuthave neverbeenexploitedforexpressionalstudiespreviouslyinPakistan.Immunohistochemistry(IHC)andinsitu hybridization(ISH)inFFPEbiopsytissuesremainsthegoldstandardforprovingEBVrelationshipina histopathologicallesionbuttheirreagentsassociatedlimitationsconfinestheirreliabilityinsomeapplications. Recentlyintroducedtargeteddrugdeliverysystemsinducevirallyticgeneexpressionandthereforerequiremore sensitivemethodtoquantifyviralaswellascellulargeneexpression. Methods:Eight(8)lymphomasampleswerescreenedtodetecttheEBVgenome.Qualitativeandquantitative expressionofEBVEncodedRNAs(EBER1,EBER2)andanti-inflammatorycytokine(interleukin-10)inFFPEEBV positivelymphomatissuesampleswerethenanalysedbyusingReversetranscriptasePolymeraseChainReaction (RT-PCR)andRealTimePolymeraseChainReaction(qRT-PCR),respectively. Results:Inthisstudywehavesuccessfullyquantifiedelevatedexpressionallevelsofbothcellularandviral transcripts,namelyEBER1,EBER2andanti-inflammatorycytokine(IL-10)intheFFPEBurkitt’slymphoma(BL) specimensofPakistaniorigin. Conclusions:TheseresultsindicatethatFFPEsamplesmayretainviralaswellascellularRNAexpression informationatdetectablelevel.Toourknowledge,thisisfirststudywhichrepresentselevatedexpressionallevels ofEBER1,EBER2andIL-10inFFPEtissuesamplesofBurkitt’slymphomainPakistan.Theseobservationswill potentiallyimprovecurrent