沼泽红假单胞菌生物转化丹参醇提物转化酶初步定域.pdfVIP

中国药物与临床2014 年9 月第 14 卷第9 期Chin晴se Remedies Clinics ，Se~mher2014，Vo1.l4，Nι9 • 1157 • ·舆验研究· 沼泽红假单胞菌生物转化丹参醇提物 转化酶初步定域 耿子颖邓欢欢梁丰色辉张源毕磊杨官生我 [摘要] 目的 比较丹参醇提物经沼泽红假单胞菌以及其各细胞部位转化后化学成分的变化，对其转 化酶在细胞部位水平上进行初步定域。方法 利用沼泽红假单胞菌各细胞部位分别对丹参醇提物进行生物转 化，高效液相色谱法(HPLC)分析转化前后主要化学成分的变化，用主成分分析法(PCA)对转化后变化程度进行 评估。结果 经不同细胞部位转化后，丹参醇提物的化学成分均发生变化。与对照比较，经细胞质、细胞膜化转后 化学成分改变最大，表明细胞质、细胞膜含转化酶更多。结论 沼泽红假单胞菌对丹参醇提物转化酶主要分布于 细胞质、细胞膜上= 【关键词 l 红假单胞菌属;丹参;生物转化;沼泽红假单胞菌:细胞部位;转化酶 Preliminary 1侃alizatiOD or RJwdopse刷品。monas palustris tamhiool extract CODvertase Geng Ziying, Deng Hua础unn， L阳ng Yanhui, zhang Yunn, Bi Lei, Yang Guone. School (扩Pluumacy， S.阳刚i Medical University, T.ω沪剧n 03α)()l ， China (Abstract) Objective To compare the differences in chemical compositions of tanshinol extract of Salvia müti- 仰hizha (S. miltiorrhiz阳) bunge following conversion by Rhodopseudorrwnω palustris (R. palωtris) and 由 individual cell organ，皿d to detemùne cellular location of tanshinol extract convertase. Meth叫s We perfonned bioconversion of the tanshinol extract using different cellular organs of R.pal.ωtris. High-perfonnance liquid chromatography was em- ployed to 路sess the changes of m勾or chemical compositions before and after the bioconver百ion. We next applied principal component analysis 伊CA) to evaluate the magnitude of variation in the chemical compositions. 四Jlts Following conversion by different cellular organs, there was significant change in all chemical components in the tan- shinol extract of S. miltωrrhizha bunge. Compared wi由 control group, lransfonnation using the cytopl幽m and cellular membrane result