猪链球菌BALBc小鼠感染模型的初步建立.pdfVIP

中国人兽共患病学报 418 Chinese J ournal of Zoonoses 2011. 27 (5) 文章编号: 1002-2694(2011 )05 一 0418-05 猪链球菌BALB/c 小鼠感染模型的初步建主骨 张奕强l·2 ，刘文倩l ，刘永生i ，王 ~p2 ，姚学萍2 ，张 杰I 摘 要:目的 观察猪链球菌(Streρtococcus suis )感染小鼠后的l临床、病理变化，为将小鼠作为实验室研究猪链球菌的实 验动物提供依据。方法 用猪链球菌活菌经腹腔注射感染小白鼠，并做阴性对照，观察其临床症状，剖解病死小鼠，制作病理 切片观察病理变化。并对病死小鼠做病原分离，通过培养特性鉴定、生化试验以及 PCR 鉴定确定其为猪链球菌。结果 小鼠 确由感染猪链球菌而致死，体内各组织、器官均产生典型的败血症病变。结论 小鼠对猪链球菌易感，能够作为实验室研究 猪链球菌良好的实验动物。 关键词:猪链球菌 ;BALB/c 小鼠模型s 病理变化 中图分类号:S852.61 + 1; R535 文献标识码:A Experimental animal models of BALB / c mice infected with Streptococcus suis ZHANG Yi-qiang 1 Z , LIU Wen-qian 1 , LIU Yong-sheng 1 , W ANG Yin2 ,Y AO Xue-ping2 ,ZHANG lie1 • (1. State Laboratory 0/ Veterinary Etiological Biology , Key Laboratory of Animal Virology of Ministry of Agriculture , Key Laboratory of Veterinary Public Health of Ministry of Agriculture , Lanzhou Veterinary Research Imtitute , Chinese Academy of Agricultural Sciences , Lanzhou 730046 , China I 2. Laboratory of Animal Qzωranti时，College of Animal Medical ,Sichuan Agricultural University ,Ya an Sichuan 625014 ,China) ABSTRACf: Mice were infected with living Stre户tococcus suis by celiac injection and the dinical s归丑ptorns were observe. The ca- davers of the infected mice were anatomized to observe the pathologic changes through pathologic section preparatio凡 According to the results of the cultural characteristics , biochemistry properties and PCR verification , the strain was identified to be Stre如何occus suis. Then the mice were died from the infection of Streρtococcus suis. Allorgans and tissues of the mice presented typical septicemic patho logic changes , proving that mouse could be a g∞d material for study of Streρtococcus .~uu. KEY WORDS: Strept町occus suis; BALB/ c mice mo