虎杖果实中白藜芦醇与大黄素提取工艺优化及其与3类根茎含量比较.pdfVIP

12 201 6, Vo1.3 7, No. lO 自由融事 ※王艺技术 虎杖果实中白黎芦醇与大黄素提取王艺优化及其 与3 类根茎含量比较 董爱文，姚妹凤，许新军，成江 (吉首大学林产化工工程湖南省重点实验室，湖南张家界 427仪)()) 摘 要z 目的:研究虎杖果实中白薯芦醇与大黄素提取工艺，比较其果实与雄株、未结果雌株、结果雌株3 类根茎 中白事芦醇与大黄素得率。方法:采用高效液相色谱法测定其果实与3 类根茎中白事芦醇与大黄素含量，确定果实 中白薯芦醇与大黄素提取的溶剂与方法，采用正交试验法考察微波火力档、溶刑体积分数、料液比及提取时间对提 取工艺的影响，确定微波辅助提取的最佳提取工艺，比较虎杖果实、 3 类根茎中二者得率。结果z 正交试验与方差 分析得出果实中白黎芦醇与大黄素的最佳提取条件为乙醇体积分数70%、料液比1:20 (g阳L)、额定功率8∞w微 波炉先40火力处理3 minì令却后60火力处理3 min、提取时间6min，高效液相色谱法测得虎杖雄株、未结果雌株根茎 中的自黎芦醇与大黄素得率均高于结果雌株根茎及果实，果实及3 类根茎中白黎芦醇与大黄素得率均超过《中国药 典)) (2010) 规定值，提取工艺合理、稳定、可行，虎杖果实具备开发利用的价值。 关键词:虎杖:自黎芦醇:大黄素:提取工艺:高效液相色谱 Optimization of Simultaneous Extraction of Resveratrol and Emodin from Fruits of Polygonum cu早idaωmand Comparison of Their Contents with Those of Rhizomes of Male and Female Plants DONG Aiwen , YAO Shufe吨， XU Xinjun , CHENG Jiang (Key Laboratory of Hunan Forest Products and Chemical Indus町Engineering， Jishou University , Zhangjiajie 427ω0， China) Abstract: Objective: To investigate th晴 simultaneous extraction of resveratrol and emodin from 由e 仕uits of Polygonum cuspidatum and to ∞mp缸e their contents with those of the rhizomes of male , non-bearing and bearing female plants. Methods: High performance liquid chromatography (HPLC) was used to determine the contents of resveratrol and emodin. The microwave-assisted extraction of resveratrol and emodin was carried out using aqueous ethanol as extraction solvent. Microwave firepower, ethanol concentration, solid-to-solvent ratio and extraction time , all of which were found to infiuence extraction efficiency, were optimized by using orthogonal 町ay experiments. Comparison of t