小檗碱对严重腹腔感染时肠屏障功能障碍的防治作用.pdfVIP

· 1 9 1 · 中华普通外科学文献（电子版）2014 年 6 月第 8 卷第 3 期 Chin Arch Gen Surg（Electronic Edition)，June 2014，Vol.8，No.3 ·论著 · 小檗碱对严重腹腔感染时肠屏障功能障碍的 防治作用 罗育其 吴承堂 于海涛 董栋 【摘要】 目的 探讨小檗碱对严重腹腔感染导致肠屏障功能障碍的防治作用 。方法 制备严 重腹腔感染大鼠模型即盲肠结扎 + 穿孔模型（CLP 模型），将 90 只 SD 大鼠随机分为感染组（CLP 感 染，不予治疗）、对照组（只剖腹而不行 CLP 术）和治疗组（盐酸小檗碱 300 mg/kg 灌胃给药）， 各 30 只，观察各组肠道病理改变，进行血细菌培养，检测血清内毒素、二胺氧化酶（DAO）水 平、D- 乳酸含量评估其肠屏障功能；同时检测血清 C 反应蛋白（CRP）、白介素 2（IL-2）、 肿瘤坏死因子 α（TNF-α）水平等炎症相关因子，了解炎症反应情况 。结果 治疗组肠黏膜损 伤明显减轻；在术后第 1、2、4 天检测点上，治疗组血清内毒素、DAO 及 D- 乳酸水平明显低于 感染组（P 0.01）。同时治疗组 TNF-α、血清 CRP 及 IL-2 水平明显低于感染组（P 0.01）。 结论 严重腹腔感染时过度炎症反应可导致肠屏障功能障碍，小檗碱通过降低炎症反应而实现对肠 屏障功能的保护 。 【关键词】 小檗碱；肠屏障功能；严重腹腔感染；炎症反应 Effect of Berberine on intestinal barrier dysfunction in rats with severe abdominal infection Luo Yuqi*, Wu Chengtang, Yu Haitao, Dong Dong. *Department of General Surgery of Guangzhou Nansha Central Hospital, Guangzhou 511457, China Corresponding author: Luo Yuqi, Email 【Abstract】 Objective To discuss the preventive and treatment effect of Berbrine on intestinal barrier dysfunction in rats with severe abdominal infection. Methods Ninety model rats of severe abdominal infection were prepared for cecal ligation and puncture (CLP). They were randomly divided into three groups: the severe abdominal infection group (prepared by CLP), the control group (laparotomy only) and the Berberine treatment group. The changes of intestinal pathology in each group were observed, blood culture and plasma endotoxin, DAO, D-lactate were taken at different times after the operation. Meanwhile, the inflammatory factor, such as TNF-α, CRP and IL-2 were tested for the inflammatory level. Results The damage of intestinal mucosa in CLP group was more severe than that in Berberine treatment group, and pla