泰山四叶参多糖对幼年小鼠和免疫抑制小鼠的免疫调节作用.docVIP

泰山四叶参多糖对幼年小鼠和免疫抑制小鼠的免疫调节作用 作者：王德才，邱玉玉，张显忠，高丽君 【摘要】 目的观察泰山四叶参多糖（PCL）对幼年小鼠和免疫抑制小鼠的免疫调节作用。方法将小鼠随机分为生理盐水对照组、环磷酰胺（CTX）对照组、PCL组（200 mg·kg-1·d-1）和CTX与PCL高、中、低剂量（100，200 ，400 mg·kg-1·d-1）联用组，连续ig 14 d，检测胸腺和脾脏指数、巨噬细胞吞噬功能以及血清特异性抗绵羊红细胞（SRBC）抗体IgM，IgG水平；并检测0.5～16.0 mg·ml-1等比设计的6个PCL浓度对体外培养的小鼠脾淋巴细胞增殖的影响。结果PCL能显著提高幼年小鼠胸腺指数和脾脏指数、巨噬细胞对鸡红细胞（CRBC）的吞噬率和吞噬指数，提高血清抗SRBC抗体水平，促进脾淋巴细胞在体外的增殖能力；呈剂量依赖性地拮抗CTX对小鼠的免疫抑制作用。结论PCL对幼年小鼠和免疫抑制小鼠的非特异性和特异性免疫功能均有明显的增强作用。 【关键词】 泰山四叶参； 多糖； 环磷酰胺； 免疫调节 　　Abstract：ObjectiveTo investigate the immunoregulation of polysaccharides extracted from Radix Codonopsis Lanceolatae from Mount Taishan(PCL) in juvenile and immunosuppressed mice. MethodsThe juvenile mice were divided randomly into six groups,namely, normal saline control, cyclophosphamide(CTX)control, PCL(200mg·kg-1·d-1）group,and three combination groups(CTX and 100，200 ，400mg·kg-1·d-1 PCL). After given orally for two weeks, the thymus and spleen index, the celiac macrophage ability of engulfing CRBC, and the antibody level of serum against SRBC were determined. Effects of six concentration levels of PCL on spleen lymphocyte proliferation were observed. ResultsCompared with the control group, in juvenile mice, PCL could increase the thymus and spleen index, enhance the phagocytic ability of the macrophage, increase the antibody level, and promote the spleen lymphocyte proliferation. In immunosuppressed mice induced by CTX, PCL could improve the immunologic indexes, counteract the immunosuppression of CTX with dose-dependence. ConclusionPCL can enhance non-specific and specific immunity of both of the juvenile mice and the immunosuppressed mice. 　　Key words：Radix Condonopsis Lanceolatae from Mount Taishan; Polysaccharides; Cyclophosphamide; Immunoregulation 　　四叶参Codonopsis lanceolata Benth.et Hook.F.又名轮叶党参、羊乳、山胡萝卜等，系桔梗科党参属多年生草质缠绕藤本植物，在我国主要分布于东北、西南等地区。四叶参在泰山山系也有分布，根入药，具清热解毒、止咳祛痰、补虚通乳、健身补气等功效，用于肺痈咳嗽、病后虚弱、产后缺乳等症，系泰山四大名药之一［1］。前期研究结果表明，泰山四叶参提取物具有耐缺氧、抗疲劳、抗氧化等作用［2～4］。为探讨四叶参提取物中活性成分的作用，本实验从泰山四叶参中提取分离多糖并观察其对幼