浅谈对2型糖尿病大鼠睾丸的IL-1β-TNF-α损伤与灵芝孢子粉的干预.docVIP

浅谈对2型糖尿病大鼠睾丸的IL-1β\TNF-α损伤与灵芝孢子粉的干预 【摘要】 目的 观察灵芝孢子粉对2型糖尿病大鼠睾丸IL-1β、TNF-α的影响。方法 Wistar雄性大鼠50 只，随机分成3组(正常组、模型组、灵芝组)。采用放免法检测睾丸IL-1β、TNF-α的含量。结果 糖尿病组与正常对照组比较，IL-1β、TNF-α含量明显高于对照组(P0.05)。而灵芝组与糖尿病组比较，IL-1β、TNF-α含量明显低于糖尿病组(P0.05)。结论 灵芝孢子粉能有效降低IL-1β、TNF-α对糖尿病大鼠生殖系统的毒性损害，对糖尿病大鼠生殖系统的细胞凋亡有明显抑制，从而是对睾丸细胞起保护作用的机制之一。灵芝孢子粉作为一种治疗糖尿病的辅助用药，在临床应用上具有广阔前景。 【关键词】 2型糖尿病；睾丸；IL-1β；TNF-α；灵芝孢子粉 　　 　　Ganoderma lucidum spre powder on type 2 diabetes rat testis IL-1β，TNF-α expressioninfluence 　　 　　ZHONG Li-li,ZHANG WEi-jia，YIN Li-ying,et al. 　　Harbin City, HEIlongjiang Province First Affiliated Hospital of the University of Chinese Medicine, Harbin 150040,China 　　 　　【Abstract】 Objective To observe ganoderma lucidum spore powder perishes weakly on type 2 diabetes big mouse testis cell IL-1β、TNF-α expression influence. Methods 50 Wistar big mouse randomly were divided into 3 groups control group, diabetes group, ganoderma lucidum group. Radioimmunoassay examines testis cell factor IL-1β、TNF-α levels.Results Diabetes group and the normal control group, IL-1β and TNF-α were significantly higher(P0.05). The ganoderma lucidum group and diabetes group，IL-1β、TNF-α levels significantly lower than diabetes group(P0.05). Conclusion The ganoderma lucidum spore can effectively reduce the IL-1β, TNF-α levels in diabetes rats toxic damage to the reproductive system,probably in diabetes rats reproductive system of apoptosis significantly inhibition of testicular cells so protective effect from one of the mechanisms. Ganoderma lucidum spore as a complementary treatment of diabetes treatment in clinical applications, has broad prospects. 　　【Key words】 Type 2 diabetes； Testis； Interleukin(IL-1β)；Tumor necrosis factor-alpha(TNF-α)；Ganoderma lucidum spore powder 　　 　　 　　糖尿病伴性功能与生殖功能障碍无论在人还是在实验动物都有报道,发病率高达35%~75%,严重影响了患者的生活质量,甚至不育,其发病机制仍未完全阐明。为探讨细胞因子在糖尿病大鼠睾丸损伤中的作用,我们通过检测糖尿病大鼠睾丸IL-1β、TNF-α含量的变化,来观察糖尿病大鼠睾丸细胞因子水平,为阐明糖尿病性与生殖功能障碍的机制奠定基础。灵芝孢子粉对糖尿病大鼠睾丸具有保护作用,从而提高性与生殖功能。 　　1 材料与方法 　　1.1 药品与试剂 　　链脲佐菌素（STZ）为北京拜尔