溶血性、非溶血性肠球菌的毒力差异.docVIP

溶血性、非溶血性肠球菌的毒力差异 【关键词】 肠球菌属;,溶血素类;,毒力;,药物耐受性 　　摘要:目的 探讨肠球菌溶血素与毒力的关系。 方法 比较溶血性肠球菌在临床标本及健康人群粪便标本的检出率;比较溶血性、非溶血性肠球菌对小鼠的LD50及其对9种抗生素的敏感性。结果 临床标本肠球菌的溶血素检出率61.5%,高于健康人群粪便标本(18.4%,P0.05);溶血性肠球菌的LD50[(3.9~11)×108cfu/mL]比非溶血性肠球菌小[(2.5~8.9)×1010cfu/mL,P0.01]。除丁胺卡那、万古霉素外，溶血性肠球菌对抗生素的耐药性明显高于非溶血株。结论 肠球菌的溶血素可能与其毒力有关。 　　关键词:肠球菌属; 溶血素类; 毒力; 药物耐受性 　　Difference of Enterococci Virulence between Hemolytic and Non Hemolytic Strains 　　Qiang Hua, Xie Manling 　　Division of Etiology, Fujian Medical University, Fuzhou 350004, China 　　ABSTRACT: Objective To investigate the relation between enterococcus hemolysin and its virulence. Methods Incidence of hemolysin of enterococci derived from clinical isolates and healthy people fecal specimens were examined respectively; LD50 and antibiotic susceptibilities were compared between hemolytic strains and non hemolytic strains. Results　Strains derived from clinical isolates exhibited higher(61.5%) incidence of hemolysin than that from healthy people fecal speciments(18.4%, P0.05). The LD50 of hemolytic strains [(3.9~11) 108cfu/mL] were at least 10fold higher than those of nonhemolytic strains[(2.5~8.9) 1010cfu/mL], P0.01. Hemolytic strains were found to be more resistant to all the 9 antibiotics tested, except amikacin and vancomycin. Conclusion These results implied that hemolysin may contribute to the virulence of enterococci. 　　KEY WORDS: enterococcus; hemolysins; virulence; drug tolerance 　　肠球菌已成为院内感染的第二位主要原因[13],其致病因子的 研究 十分迫切与重要。有研究表明,肠球菌溶血素在眼内炎、心内膜炎、腹膜炎等动物模型中有致病作用[46]。笔者比较溶血性肠球菌、非溶血性肠球菌对小鼠的LD50及其对抗生素的敏感性,通过 医院 临床标本以及健康人群粪便标本肠球菌溶血素的检出率调查,探讨溶血性、非溶血性肠球菌的毒力差异以及溶血素在肠球菌的毒力作用。 　　1 材料与方法 　　1.1 材料 　　1.1.1 菌株 　　1.1.1.1 肠球菌菌株 　　临床菌株分离自福建医科大学附属协和医院及福建省立医院就诊患者的尿液、胆汁、腹腔液和心瓣膜、白带、前列腺等临床标本,健康人群菌株分离自某宿舍区健康自愿者、福建医科大学教职工、学生等粪便标本。 　　1.1.1.2 质控菌株 　　药敏质控菌:肠球菌ATCC29212(敏感株)、肠球菌ATCC51299(耐药株)、金黄色葡萄球菌ATCC25923、大肠埃希氏菌ATCC25922(卫生部药品生物制品检定所)。溶血对照乙型链球菌Bd1、甲型链球菌Bc3为福建医科大学病原生物学系保存菌株。 　　1.1.2 动物 　　出生4周昆明种小鼠,体质量(19±1)g,雄性,清洁级(闽实动质准第00205),福建医科大学实验动物中心提供。 　　1.1.3 培养基 　　1.