电针治疗对大鼠脊髓损伤后Nogo_A蛋白表达的影响.docVIP

电针治疗对大鼠脊髓损伤后Nogo_A蛋白表达的影响 作者：李振鹏，孔抗美，陈育春，郭天明，陈泽锋，刘加贝 【摘要】 目的：探讨电针 治疗 对大鼠脊髓损伤(SCI后Nogo_A蛋白表达的 影响 。 方法 ：以96只2月龄Wistar大鼠为受试对象，SCI组于T9处行脊髓全横断，治疗组予电针治疗，采用Western blot测定各时间点及各组脊髓Nogo_A表达及变化,并以苏木精_伊红和免疫组化染色对受损脊髓进行形态学观察。结果：SCI后Nogo_A蛋白表达呈递增趋势，于14d后最强；经电针治疗，Nogo_A表达减少。结论：Nogo_A是SCI后脊髓神经纤维再生的主要抑制因子，电针治疗对Nogo_A表达具有明显的抑制作用。 【关键词】 电针；脊髓损伤；Nogo_A蛋白 ［Abstract］Objective: To study the effect of electroacupuncture on the expression of Nogo_A protEin in rats with spinal cord injury(SCI. Methods: Ninety_six adult Wistar rats were randomly assigned into control group(A, SCI group(B, sham_treatment group(Cand electroacupuncture treatment group(D. Group B, C and D were received transect SCI at the T9 vertebrae level while group A was just opened the vertebra and their spinal cords were not wounded. Group D was treated with electroacupuncture. All the spinal cords of the trauma section were taken out at different time points after o_peration. Western blot and immunohistochemical methods were used to examine the change of the expression of Nogo_A in these tissue. Results： Just minim Nogo_A was found in the control group, while the manifold expression of Nogo_A was found after operation，especially at the end of the second week, the expression was inhibited by electroacupuncture. Conclusion: Nogo_A is a major suppressor factor of spinal cord nerve fiber regeneration after SCI, and the electroacupuncture can obviously inhibit the expression of Nogo_A protein. ［Key Words］electroacupuncture，spinal cord injury，Nogo_A protein 脊髓损伤(SCI临床常见，致瘫率较高。电针是治疗SCI的重要方法,其疗效已获得临床实践和动物实验［1_2］的证实，但其作用机制尚未完全明确。本实验通过电针治疗SCI大鼠，于不同时间点采集大鼠受伤脊髓段，以Western blot测定其Nogo_A蛋白表达，探讨电针治疗SCI的机制。 1 材料与方法 1.1 动物模型制作 Wistar大鼠(广东省实验动物中心提供96只(2月龄，体质量220～270ｇ，雌雄各半，随机分为对照组(A组。24只和SCI组(72只。SCI组于T9处行脊髓全横断，然后再随机分为损伤组(B组，硬膜外腔不留置电极、治疗对照组(C组，留置电极但不通电和电流刺激治疗组(D组，损伤部位上下端的椎间隙硬膜外腔留置电极，予G6805_2型多用治疗仪治疗，强度以大鼠有轻微肌肉抽动和无明显不安嘶叫为适，每天治疗30min，疗程至处死动物取标本前为止，各组24只。各组分别于术后1、7、14和28d取各损伤部位的脊髓组织(每组3只置_70℃冰箱保存备用。 1.2 Western blot测定Nogo_A蛋白