胃癌组织中P16，CMYC和COX2的表达及其与患者预后的关系.docVIP

胃癌组织中P16，CMYC和COX2的表达及其与患者预后的关系 作者：翟俊山 李楠 张林 王艳梅 朱超慧 吴凯 【摘要】 目的］探讨胃癌组织中P16，CMYC和COX2表达情况、相互关系及其与患者预后的关系。［方法］应用免疫组化SABC法检测104例手术切除胃癌组织中P16，CMYC和COX2的表达。［结果］胃癌中P16，CMYC和COX2表达阳性率分别39.4%(41/104)，57.7%(60/104)，63.5%(66/104)。P16的表达在胃癌中与不同的浸润深度，淋巴结有无转移，TNM分期，不同组织学类型均有显著差异(P＜0.05)；CMYC的表达在胃癌中与不同的浸润深度，淋巴结有无转移，TNM分期有显著差异(P＜0.05)，而与不同组织学类型无统计学差别(P＞0.05)；COX2的表达在胃癌中与淋巴结有无转移，TNM分期，不同组织学类型均有显著差异(P＜0.05)，而与不同的浸润深度无统计学差别(P＞0.05)。而它们与肿瘤患者年龄，性别均无统计学差异(P＞0.05)。等级相关分析结果显示P16与CMYC、COX2表达呈负相关(P＜0.05)，CMYC与COX2表达呈正相关(P＜0.05)。生存曲线研究结果显示P16，CMYC和COX2表达与患者术后生存时间显著相关(P＜0.05)。［结论］P16在胃癌组织中低表达及CMYC和COX2高表达能客观反映胃癌组织分化程度，侵袭转移能力，且其与患者预后密切相关。故检测三种指标可正确判断胃癌患者预后，在指导临床 治疗 方面有一定价值。 【关键词】 P16；CMYC；COX2；胃癌；预后；基因表达 　　Abstract：［Objective］To investigate the expression of P16，CMYC and COX2 in gastric carcinoma and relationship with the pathogenesis，infiltration，metastasis，prognosis of the patients.［Method］ The expressions of P16，CMYC and COX2 were detected by inununohistochemical methods in 104 surgical resection specimens.［Result］The positive expression rates of P16，CMYC and COX2 were 39.4%(41/104),57.7%(60/104),63.5%(66/104).The expression of P16 was notably correlated with the depth of invasion，lymph nodes metastasis，pathologic type，TNM stage in gastric carcinoma (P＜0.05)；the expression of CMYC was notably correlated with the depth of invasion，lymph nodes metastasis，TNM stage in gastric carcinoma (P＜0.05),but not related to the pathologic type(P＞0.05)；the expression of COX2 was notably correlated with lymph nodes metastasis，pathologic type，TNM stage in gastric carcinoma (P＜0.05),but not related to the depth of invasion(P＞0.05)；they all were not related to the age and sex (P＞0.05).The expression of P16 was negatively correlated with CMYC and COX2 protEin (P＜0.05).The expression of CMYC protEIn was positively associated with that of COX2. Also，the expressions of P16，CMYC and COX2 were obviously correlated with the survival time of postoperative patients (P＜0.05).［Conclusion］ The lower expres