胎盘小叶胎儿血管系统促肾上腺皮质激素释放激素及其受体CRH-R1的表达.pdfVIP

胎盘小叶胎儿血管系统促肾上腺皮质激素释放激素 及其受体 CRH-R1 的表达 王晓东，余海燕，姚 强，刘淑芸Δ 四川大学华西第二医院妇产科 （成都 610041 ） [摘要] 目的 了解足月胎盘小叶胎儿血管系统促肾上腺皮质激素释放激素(CRH)的表达。方法 分别 采用免疫组织化学技术 LsAB 法和原位杂交技术，观察先兆临产前人足月正常胎盘小叶血管 CRH 及其受体 CRH-R1 的表达。结果 胎盘小叶血管内皮细胞、血管平滑肌细胞的细胞浆内均有 CRH / CRH-R1 阳性表 达，同时也有 CRH mRNA / CRH-R1 mRNA 阳性表达。结论 胎盘小叶脐血管系统血管内皮细胞甚至平滑 肌细胞能合成 CRH 及 CRH-R1，其 CRH 可能是胎儿血 CRH 的主要来源之一。 [关键词] 胎盘小叶 促肾上腺皮质激素释放激素 胎儿缺氧 [中图分类号] R714.56 Expression of corticotropin-releasing hormone and its receptor type-1 in the placental cotyledon vessels WANG Xiaodong, YU Haiyan, YAO Qiang, LIU Shuyun. Department of Obstetrics and Gynecology, West China Second Hospital of Sichuan University, Chengdu 610041, China Corresponding author: LIU Shuyun, 610041 Chengdu, Department of Obstetrics and Gynecology, West China Second Hospital of Sichuan University, China [Abstract] Objective To study expression of corticotropin-releasing hormone and its receptor type-1 in the placental cotyledon vessels at term. Methods This study has used IHC-SP staining and ISH methods to investigate the expression patterns of CRH and its receptor CRH-R1 in the placental cotyledon vessels at term from women who have undergone uncomplicated pregnancies. Placental tissues were collected after cesarian section before threatened labor. Results Our study shows for the first time that endothelial cells and even smooth muscle cells of the cotyledon vessel express CRH messenger ribonucleic acid and peptide as well as the CRH-R1. Conclusion Novel findings are presented on that CRH derived from endothelial