chap five gene transfer课件.pptVIP

Chapter 5 transfer the aim gene into the receptor cell Section one receptor cell 受体细胞：可摄取外源DNA并使其稳定维持的细胞，具有应用价值或理论价值。 又称宿主细胞或寄主细胞， The qualification for the cell to be receptor cell： It is Easy to be transferred with recombination DNA. The recombination DNA can exist in the cell stably. Easy to select the recombinant(重组体)。 Genetic characters of the cells are stable,the cells can be cultured easily. safe，without pathogenicity,could not pollute the environment. Without proteinase or it’s content is very low, so the protein expressed from the foreign gene can accumulate 遗传密码的应用无明显偏奇性。 有较好的转译后加工