烟草赤星病菌(Alternarialongipes)产角质酶液体发酵-中国烟草科学.PDF

58 中国烟草科学 2009 ，30 （5 ）：58-61 烟草赤星病菌(Alternaria longipes)产角质酶 液体发酵条件的优化 ﹡ 刘 辉，刘爱新，梁元存 ，刘 菲，魏芳芳 （山东农业大学植物病理系，山东泰安 271018 ） 摘 要：以基础培养基（查彼培养基＋碳酸钙＋苹果角质）为营养优化了长柄链格孢(Alternaria longipes)产角质酶的环境 和营养条件。结果表明最佳产酶的环境条件是：培养时间8 d，培养温度25 ℃，pH 5.5 ；通过正交试验确定最佳产酶的营养 条件是：蔗糖3 ％，干酪素1 ％，吐温-80 0.3%；酶活高达175.2 U/mL，是基础培养基酶活力的11.0 倍。 关键词：烟草赤星病菌；角质酶；发酵条件 中图分类号：S435.72 文章编号：1007-5119 （2008 ）05-0058-04 DOI: 10.3969/j.issn.1007-5119.2009.05.013 Optimal Fermentation Condition of Producing Cutinase by Alternaria longipes LIU Hui, LIU Aixin, LIANG Yuancun ﹡, LIU Fei, WEI Fangfang (Department of Plant Pathology, Shandong Agricultural University, Taian, Shandong 271018, China) Abstract: Alternaria longipes is the causal agent of tobacco brown spot, a world-wild tobacco disease. Based on Czapek medium supplemented with CaCO and apple cutin, the liquid fermentation conditions were optimized in order to acquire higher cutinase 3 activity. Optimum culture time, temperature and pH were 8 d, 25℃ and 5.5, respectively; and optimum carbon and nitrogen sources were dextrose and maltose instead of NaNO . According to this fermentation and orthogonal test, the optimum composition of culture 3 medium included dextrose 3 ％, casein 1 ％, Tween-80 0.3%. A 11.0-fold higher cutinase activity was obtained compared to the basal medium and the enzyme activity was enhanced from 15.8 U/mL to 175.2 U/mL. Keywords: Alternaria longipes ; cutinase; fermentation condition 角质酶是丝氨酸酯酶的一种，可催化水解不溶 fusca 产角质酶摇瓶发酵条件进行了研究[11] ，之后 性角质的酯键，不同真菌来源的角质酶相对分子质 同一研究室又对此菌的高产角质酶突变株进行了