纤维素降解菌简青霉H11的筛选及酶学特性的研究-华北农学报.PDF

华北农学报2007 , 22 ( 3) : 160- 162 H11 白洪志, 杨 谦, 张鲁进 ( , 150001) : , , , : CMCase pH 28, 50 , 30~ 50 FPase pH 48, 60 , 30~ 40 : ; ; :TQ34 : A : 1000- 709 1( 2007) 03- 0160- 03 Study on Selection and Enzymatic Properties of Penicillium simp licissimum H11 Producing Cellulase BAI H ngzhi, YANG Qian, ZHANG Lujin ( Department f Bi science and Techn l gy, Harbin Institute f Techn l gy, Harbin 150001, China) Abstract : A strain pr ducing cellulases were selected and is lated fr m wide f rest s il by cultured n plates f C n- g red dying, in liquid medium with filter paper, then fermented in liquid medium. Different pH and temperature and were perf rmed t find the ptimal c nditi n f enzyme activity. The results sh wed that the ptimal reacti n c nditi ns f CMCase were 50 , pH 28, the enzyme activity was very stable under the temperature ranging between 30- 50 The ptimal c nditi ns f r the enzyme reactivity f FPase were pH 48, 60 , the enzyme activity was very stable under the temperature ranging between 30- 40 Key words: Penicillium simp licissimum; Cellulase; Enzymatic pr perties ( Cellulase) , CMCDNS [5] 1, 4 , [ 1- 3] ( NH4 ) 2SO4 30 g, FeSO4 7H2O 0005 g, KH PO 10 g, MnSO H O 0001 6 g, MgSO 7H O , 2 4 4 2 4 2 [4] 05 g, ZnSO 7H O 0001 7 g, CaCl 01 g, C Cl 0002 4 2 2 2 , g, NaCl 01 g, 20 g, 20 g, 1 000 mL ( Peni-