45110301蒲晓聪.ppt

* 请在此页填写作品信息（此页非设计模板） 主办方 作者学号 作者姓名： 蒲晓聪 Research and Application of Cyclodextrin glycosyltransferase 2.What is the cyclodextrin glycosyltransferase 1.What is the cyclodextrin？ Cyclodextrin，CD, is by 12 D-glucopyranosylwith α- l, 4 glycosidic bond-glucose Connected cyclic oligosaccharides Cyclodextrin Glycosyltransferase,CGTase , Acting on starch ，synthesis a one non-reducing maltooligosaccharide with other α-1,4glucan 。 目录页 （设计好之后可以删掉这个文本框哦） 1、Research of CGTase 1.1、CGTase’s Producing strain B.maceran、B.stearothermophilis B.megateriu、B.lichenrmis、 B.circulars、B.subtiIisV、B.firmus、 Klebl.sellapneumonl.a and so on。 Currently，B.stearothermophilis commonly used in the industrial production of bacteria 。 1.2、Breeding of CGTase’s Producing strain Mutation breeding，including physical mutagenesis and Chemical mutagenesis Physical mutation commonly used ultraviolet mutagenesis, laser, and can lead to ionizing radiation X-ray, Y-ray, fast and microwave mutagenesis。 Chemical mutagenesis method and reagent sort is more, mainly include all kinds of alkylating agent such as cool methyl sulfonic acid b, a heartland of nitro vein, diethyl sulfate and Light amine hydrochloride. 1.3.Enzymology characteristics of CGTase For basophilic bud holding coli N-227 strains, to pass through the CaCI: fermented liquid handling, acetone precipitation, systemCrude enzyme preparation. Enzymology experiments showed that the optimum pH 4.0-9.0; Under the condition of 60 degrees Celsius, pH5.0-9.0, enzyme activity is relatively stable optimum temperature of 60 degrees Celsius, 70 degrees Celsius under stable enzyme activity, calcium ions on enzyme plays a protective role 1.4、immobilization of CGTase According to the different immobilized method ，it can be divided into five categories：Physical adsorption、Ionic bond legal、The embedding method、Carrier crosslinking method、 Covalent method。 According to