发酵种子罐接种标准操纵规程(国外英文资料).doc

发酵种子罐接种标准操作规程Objective: to set up the standard operating procedure for seed canning and to train the staff to train education.Range of 2.0: the operation of the seed tank in the fermentation engineering department.Definition 3.0: no4 responsibilities: be responsible for the procedure and QA supervision.5.0 application:Preparation before vaccination:The process notifies the attendants to close the window of the seed tank in the first half hour prior to the vaccination and then spray the seed tank with 1 percent new jure.5.1.2 ready 1 percent of new jure, 75% alcohol and sterile gauze; Confirm that the receiving tank is running normally, the tank pressure is 0.03 -0.05 MPa, the tank temperature and pH are within the range of the craft requirements. The end of the inoculated silicone tube is well protected and has a live air microrow.5.1.3 is ready to use the super net, turn on the ultraviolet lamp, turn on the fan for high grade, and the ultraviolet lamp for 30 minutes. Then turn off the ultraviolet lamp and open the fluorescent lamp.After receiving the inoculation from the fungus, the technology officer checks the gas tightness (silicone, clamp, and silicone tube) in good condition.Inoculation: the operation is performed by the technologists and the attendants assist. All vaccinators should wash their hands before vaccination.5.2.1 technologizer USES 75% of the alcohol to disinfect the hands, then put on the sterile gloves and disinfect with 75% alcohol.5.2.2 the ends of the seed jar mouth end and inoculated bottle near each other, to get rid of the vaccination mouth end gauze, remove vaccination bottle silicone tube gauze, at the end will dock silicone tube mouth to mouth quickly, docking and sprinkle with 75% pure sterile gauze pinched silicone tube and vaccination junction mouth, prevent fall off in the process of according to the kind of pipe.After docking, open the squirming pump to break into the seed can.5.3.4 when there is no seed fluid in the jar of the jar,