双侧迷走神经和颈总动脉联合切断法.doc

双侧迷走神经和颈总动脉联合切断法 建立急性脑缺血大鼠动物模型的实验研究 徐彩云1 王忠2 吴凌宇1李鑫月1张大维1﹡ ( ) 摘要 目的：建立一种操作简单的急性脑缺血动物模型。方法：取雄性Wistar大鼠40只，体重在200—230g，手术前禁食12小时，自由饮水，随机分为对照组Ａ、Ｂ、Ｃ组及模型Ｄ组，共4组，每组10只。即A组：假手术组，仅切开颈部两侧皮肤，分离双侧颈总动脉和迷走神经，不切断，然后缝合；Ｂ组：仅切断双侧颈部迷走神经；Ｃ组：结扎并切断双侧颈总动脉（ＣＣＡ）；D组：联合组，即结扎并切断双侧颈总动脉（ＣＣＡ），同时切断双侧颈部迷走神经。观察各组大鼠手术后的脑缺血症状，记录各组大鼠在8小时内的死亡情况，超过8小时死亡的动物按8小时计，计算死亡率和死亡时间。结果：Ａ组大鼠没有脑缺血症状，无死亡；Ｂ组大鼠无脑缺血症状，呼吸变慢变深，心率血压上升，但无死亡；Ｃ组大鼠部分出现脑缺血症状，眼睑下垂，活动能力低下，自发运动减少，也有些大鼠术后自发运动增加，在8h内无死亡；Ｄ组大鼠大多数出现较为明显的脑缺血症状，在8小时内全部死亡。结论：采取同时结扎并切断大鼠双侧颈总动脉和双侧颈部迷走神经的方法，可以建立急性脑缺血大鼠动物模型，此方法具有手术简单，成功率高，术后动物缓慢死亡的特点。 关键词: 联合切断法;　急性脑缺血;　动物模型 Bilateral vagotomy and carotid arteries combined cutting method Establishment of rat model of acute focal cerebral ischemia animal model experimental study XU Cai-yun1，WANG Zhong 2 ，WU Ling-yu1 ，LI Xin-yue 1，ZHANG Da-wei1* 1( Jilin University, Bethune Medical University Laboratory Animal Center, Changchun130021,China;) ［作者简介］徐彩云，（1964-），女 ，实验师，大学本科，研究方向：实验动物学。 E-mail: xucy@jlu.edu.cn ﹡2( the experimental animal center of Xinjiang Medical University Urumqi 830054) Abstract：Objectives: To establish a simple acute cerebral ischemia animal model. Methods: 40 male Wistar rats, weighing 200- 230grams, all rats fasting before operation12 hours, free drinking water, were randomly divided into control group, A, B and C group, D group and model group,4,10 each. A groups: sham operation group, only cut on both sides of the neck skin, separation of bilateral internal carotid arteries and vagus nerve, not cut off, and then stitched; group B: only the section of bilateral cervical vagus nerve; group C: only ligated and transected bilateral common carotid artery (CCA ); group D: combined group, namely the ligated and transected cervical total arterial ( CCA ), at the same time section of bilateral cervical vagotomy. Rats were observed after the operation of cerebral ischemic symptoms, rats were recorded within 8 hours of death, more than 8 hours of the death of the animal in8hours, calculation of mortality and dea