严重创伤失血性休克后亚低温治疗对大鼠细胞免疫功能的影响.pdfVIP

2252 重庆医学 2007 年 11 月第 36 *-J再 22 期 ·论著· 严建制伤失血性休克后班低溜:自疗对大鼠细胞免眩功能的影响 杨事，~迹I~ {第二五 ~a且大学大坪隐院全且在战创伤中心，受 400042) 摘 :1:目的 研克亚低混治疗啡严重创萌虫血性休克政式鼠.t.戒功能的影响。方法 (1)辑用大鼠tJH事虫融体虱模型， 50 月 5D 大鼠随机命南 5 艘，分设且常付照、辄.亚低iU古咛舰、..r..常体混均持舰，粉刷 (6 、 24h) 血革中 T 淋巴如脆;11:群 (CD扩、 CD8+ ), (2) 革用体外提高盘，命南各银点鼠脾琳己制脆体外培养，观事 ConA 剌激的牌琳已捕也命措 IL-2 、 TNF咱的量t1t. 捕S属 苟且营体2自治呻舰相比，亚低混舰的 T 琳巴制胞;11:群(CDν 、 CD8+ )、 IL-2 、 TNF-a指赫虱越1..r..带，矗冉有统计学意乱(P 0.01)。精it ;II: {I民涌端#付严草创伤虫h怯休克政先鼠细胞克戎功能有…珑的保护作周. 关键词:亚低炼，严重创伤，大鼠8 克戒功能 中阁分3居曙:R365. 641 )t献栋\p，确 :A 文章E编崎: 167J.8348 ( 2007) 22-2252阳02 10ft.附nce of therapic mild hypothermja 00 jmmune tundion øf rats with be剧。rrhagic .bock indu础d by 幽咽...In如町 YANG Jun.ZHANG Lía仲yartgA (Ðeþartment 01 Trau.ma. aηd Illjuríes ,IrJStitute 01 Surgery Research ,Dapìllf(. Hosþtta. l t Third Medical Mili阳1y UnilJersíty ,ChungqÎng 400042~Chína) Abstract:Objective To investigate the Înfluenc:e of 由erapïc- mHd hypothe口nia on immune functìon of rats wíth hemorrhagíc shock induced by severe injury. Meth晒s (1 ) Usíng the traum.a and hemorrhagic 到hock model , fifty SD rats wete randotnly divíded into fíve groups , normal ccntrúl group , mîld hypùthermìa therapeutícs group(ôh.24h) and normal body tetnperature therapeutics (BTJ group(6h , 24h). W. detected the eχ_pression change of the s lIbsets of T lymphocyte (CD4伞 .CD西+)În pl a.J; ma. (2)Splenic lymphocyte cclls were isοlated and cultured. We observed the expre:s sion change of IL-2 and TNFαsecreted from the ConA-ín- duced splenic lymphocytc cel l. R剧。Its Compared with that in normal BT group. CD4+ , CD8+ , IL-2 and TNF-n in hypothermic group trended more nonnatty and had notable varíanc