通过不同途径注射可生物发光肿瘤细胞构建膀胱癌全身多器官转移模型.pdfVIP

58 中华细胞与干细胞杂志(电子版)2016年2月第6卷第1 期C恤l1 CellS阳n Cell (Electronic 剧ition)， Feb 2016，飞ToL 6,No. 1 ·论著· 通过不同途径注射可生物发光肿瘤细胞构建 膀肮癌全身多器官转移模型 未凌峰谭建明 [摘要] 目的 探讨通过不同途径注射可生物发光肿瘤细胞构建裸鼠膀肮癌全身多 器官转移模型。方法 以慢病毒感染膀肮癌细胞株5637 获得可稳定表达萤火虫荧光素酶的 5637-Luc。分别通过左心室及尾静脉注射构建膀肮癌全身多器官转移模型，并以小动物活 体生物发光成像技术观测 5637-Luc 在裸鼠体内各主要器官的分布，于 7 天后处死小动物， 取出裸鼠内脏观测转移病灶数量并进行对照分析。结果 成功构建可生物发光膀肮癌细 2 胞系 5637-Luc ，生物发光活性与细胞数高度线性相关CR = 0.995) 。经小动物活体生物发光 成像现测，通过左心室及尾静脉注射 5637-Luc 后均可构建膀肮癌全身多器官转移模型，经 左心室注射组失败2 例，经尾静脉注射组 10 {7~全部成功。 7 天后经左心室注射组平均转移 病灶数(11.9 土 0.83) ，经尾静脉注射组转移病灶数为C 1 1. 8 士 0.79) ，注射途径对于肿瘤细胞 在裸鼠体内主要器官的分布数量差别无统计学意义CP 0.05) 。结论通过不同途径注射 可生物发光肿瘤细胞均可成功构建可非侵入性、定量、实时、精确和动态观测的膀肮癌全身 多器官转移模型。 [关键词] 膀肮肿瘤: 生物发光; 肿瘤转移: 模型，动物 Establishment of metastasis model of bladder cancer by bioluminescence tumor cells via different injection routes Zhu lingfeng, Tan Jianming. Department ofUrology, Fuzhou General Hospital ofNanjing Military Command, Fuzhou 350025, China Correspondence author: Tan Jianming, Email:tanjm156@Xmu.edu.cn [Abstract 1 Objective To develop metastasis model of bladder cancer by biolu日lÏnescence tumor cells via different injection routes. Methods A human transitional cell carcinoma 5637 cellline that stably expresses luciferase by lentivirus infections was generated, and inoculated into the left cardiac ventricle or vena caudalis of immunocompromised mice. Cell distribution was monitored by IVIS200. Analysis of linear regression and t test were used in the study. Results The bioluminescence intensity was linearly correlated with cell counts CR2 = 0.995) . Cancer cells could not be detected in two mice in trans-Ieft cardiac ventricle injection group while cancer cells were detected in all mice in vena caudalis group. The metastasis lesion in thet