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* The purity and recovery of Prominin-1 positive cells is higher after myelin removal. Prominin-1 is an extreme case because prominin-1 is part of the myelin membranes. * Even for Western blot the removal of myelin has been found very useful. * * Cells in a single-cell suspension are magnetically labeled with MACS? MicroBeads. The sample is applied to a MACS Column placed in a MACS Separator. The unlabeled cells pass through while the magnetically labeled cells are retained within the column. The flow-through can be collected as the unlabeled cell fraction. After a short washing step, the column is removed from the separator, and the magnetically labeled cells are eluted from the column. Thus, with MACS Technology both labeled and unlabeled cells can easily be isolated with high purity and recovery.  * If available use direct conjugate. We now have direct conjugates for all main cell types: neurons, astrocytes, microglia, oligodendrocytes If not: Any primary antibody (titrated!!) can be used with our secondary antibody-MicroBeads. * * * (GLAST is a Glu-Asp transporter) ACSA-2 antibody against GLAST is the first extracellular monoclonal antibody for astrocytes which allows MACS. Not expressed on neurons, microglia or oligodendrocytes Fig: green GLAST, red GFAP The extracellular antibody produces a different staining in immunohisto/cyto- chemistry (pure antibody) GLAST can also be used for radial glia (early neural progenitors in the embryo), and for Müller glia in the retina and Bergmann glia in the cerebellum. In addition neural stem cells in the SVZ are GLAST-positive * High purity! After NDTK-Trypsin, enrichment from 18% to 95 % Fig Astrocytes (7 DIV) isolated from P1 mice using the Anti-GLAST (ACSA-1) MicroBead Kit. Astrocytes were stained using Anti-GLAST (ACSA-1) (green) and Anti-GFAP (red). * High purity! After NDTK-Trypsin, enrichment from 18% to 95 % Fig Astrocytes (7 DIV) isolated from P1 mice using the Anti-GLAST (ACSA-1) MicroBead Kit.