P16cyclinD1和CDK4在膀胱移行细胞癌中表达的临床意义.docVIP

P16、 cyclinD1 和CDK4 在膀胱移行细胞癌中表达的临床意义 　　作者：李庆元 刘少青 迟玉友 作者单位：滨州医学院附属医院泌尿外科 滨州市 256603 　　【摘要】 目的 探讨细胞周期调控系统相关因子在膀胱移行细胞癌中表达的临床意义。方法 应用免疫组化染色方法检测P16、 cyclinD1 和CDK4在92例膀胱癌中的表达，运用Χ2检验统计分析。结果 ① 在膀胱癌组织中P16表达下调; cyclinD1和CDK4表达上调。②92例膀胱癌中，P16保留表达率随分级、分期的增加而下降，在复发肿瘤中下降;③92例膀胱癌中，cyclinD1 和CDK4保留表达率随分级、分期的增加而增加，在复发肿瘤中增加。结论 P16、 cyclinD1 和CDK4的表达和膀胱移行细胞癌的生物学行为密切相关，在膀胱癌的发生发展中有重要意义。 　　【关键词】 膀胱移行细胞癌 细胞周期蛋白 细胞周期蛋白依赖激酶 细胞周期蛋白依赖激酶抑制因子 　　The role of expression of P16，cyclinD1 and CDK4 in transient cell cancer of bladder 　　LI Qingyuan LIU Shaoqing CHI Yuyou 　　Department of Urological ,Affiliated Hospital of Binzhou Medical University,Binzhou 256603 　　【Abstract】 Objective To probe into the relation between the expressions of P16，cyclinD1 and CDK4 in the transient cell cancer of bladder and biological behavior of the bladder cancer.methods All of specimen of 92 cases of transient cell cancer of bladder and 13 cases normal tissue were formalized, capsulated with paraffin , sliced up with interval of 4 Μm, analysised with method of HE and immunohistochemistry，examined with Χ2. Results ①Expression of P16 decreases in bladder cancer . Expression of cyclinD1 and CDK4 increases in bladder cancer .②In the 92 case of bladder cancer, expression of P16 decreases with grade and stage of tumor,expression of P16 decreases in recurring cases.③In the 92 case of bladder cancer, expression of cyclinD1 and CDK4 increases with grade and stage of tumor, expression of cyclinD1 and CDK4 increases in recurring cases.Conclusion The rate of reserve expression of P16，cyclinD1 and CDK4 in the transient cell cancer of bladder is related with biological behavior of the bladder cancer. 　　【Key words 】 transient cell cancer of bladder，P16，cyclinD1，CDK4 　　为进一步探讨其在膀胱移行细胞癌中表达的改变，本文采用免疫组化的方法系统的研究了P16 、cyclinD1 和CDK4 在膀胱移行细胞癌中的表达的临床意义。 　　1 资料与方法 　　1.1 临床资料 选择本院2000年mdash;2006年手术切除并经病理证实的膀胱移行细胞癌石蜡标本92例。男62例，女30例;年龄37～78岁，平均53岁。初发53例，复发39例;肿瘤直径≥2cm者63例，2 cm者29例;单发43例，多发49例。病理分级按WHO标准，I级