生命科学前沿讲座幻灯片.pptVIP

Cell lines derived from mammalian cells e.g. tumor cells, embryonic stem (ES) cells, transformed “normal” cells, primary cells Highly amenable to biochemical studies Not very amenable to most genetic techniques RNA interference provides quick, effective tool for gene silencing Model for RNAi mechanism RNA interference in mice Approaches for whole organism expression: Express short hairpin RNA (shRNA) in live mice: - Introduce into mouse embryonic stem cells and use standard transgenic techniques to build transgenic mice OR - Inject directly into fertilized eggs and implant in mouse uterus A