RFLP analysis(国外英文资料).docVIP

RFLP analysis(国外英文资料) The expanded condition of the test for the stain of ethyl bromide ingot. The problem and its solution should be noted (1) after the enzyme solution is done, yi2 ul is used to determine whether the enzyme solution is complete with a microelectrophoresis, to determine whether or not to end the enzyme reaction. (2) the enzyme solution must be thorough, or it will lead to incorrect genetic analysis. 5 RFLP analysis of the superiority of the phenotype Ever since Kan and Dory were first in 1978? - globin genes found in the first DNA polymorphism loci, since people have attention from product - enzyme gene expression and protein level to study the polymorphism of molecular level. This is because RFLP has many good features as a genetic marker: from DNA viruses to higher eukaryotes, RFLP is ubiquitous. In theory, using RFLP can reveal differences in the level of DNA in different genotypes. Stable heredity, the RFLP of the nuclear genome has the various genetic characteristics of Mendelian factors, and the RFLP in the cytoplasmic genome is typically characterized by maternal inheritance; Codominance: most of the RFLP bands are codominant and can easily be separated from a homozygote, resulting in more genetic information. (4) the combination of the probe with restriction enzyme amount is infinite, even if the heterologous genes can also be used as a probe, the probe can be found variation function, can also be found that the DNA of the introns and interval static variation and flanking sequence variations. 5 RFLP most is not encoded protein area and no control function of the important areas of the neutral mutation, the plant itself is usually harmless, more wont produce lethal effect, few secondary effects on major economic properties. At the same time, the RFLP is linked to the gene chain, which can be analysed by molecules without the need to determine the genetic sequence. The RFLP is not affected by the development of plants or organs, and can be