鲁凤民hcv核心抗原检测.ppt

* Anti-HCV detecting is useful for screen, and a primary assay to diagnose HCV infection as well. Anti-HCV can be detected by enzyme immunoassays (EIAs), enhanced chemiluminescence immunoassays (CIAs) or electrochemiluminescence assays (ECLIAs), either in the serum or in plasma. FIGURE: Since 1990, the anti-HCV assay has evolved from the first-generation assay to the third-generation one. The specificity of the approved anti-HCV EIAs is 99%. However, even a specificity of 99% does not provide the desired predictive value among the population with a low prevalence of infection. Among the immunocompetent populations with anti-HCV prevalences 10%, the proportion of false-positive results ranged from 15% to 60%. Therefore, samples negative in an approved EIA can be reported as anti-HCV negative and samples reactive in an approved single EIA can be reported as anti-HCV positive provided the signal-to-cut-off ratio is sufficiently high to be predictive of a true positive. However, it should be noted that individuals on hemodialysis or coinfected with HIV might be HCV RNA positive but anti-HCV negative. * * * Architect HBsAg 检测 * * Architect HBsAg 检测 * * * * Mean plasma HCV core Ag (no significant difference for HCV core Ag levels at 4°C, 20°C and 37°C between 0 and 96 h) c) Mean whole blood HCV core Ag (p0.05 for difference of HCV core Ag level at 4°C between 0 and 96 h, no significant difference for HCV core Ag at 20°C and 37°C between 0 and 96 h)“ b) Mean plasma HCV-RNA (p0.05 for difference of HCV-RNA at 37°C between 0 and 96 h, no significant difference for HCV-RNA at 4°C and 20°C between 0 and 96h)“ d) Mean whole blood HCV-RNA (p0.05 for difference of HCV-RNA at 20°C and 37°C between 0 and 96 h, no significant difference at 4°C between 0 and 96h) * * Architect HBsAg 检测 * * Hepatitis C Virus Virus first identified in 1989, Flaviviridae family Small, lipid-enveloped virus (50nm) Single, positive-stranded RNA virus----naked HCV genome infectious Envelope Core Ag ss