64例原发性胆囊癌中PTEN和c-erbB-2基因表达.docVIP

64例原发性胆囊癌中PTEN和c-erbB-2基因表达 【摘要】 ［目的］ 研究 PTEN 和c-erbB-2基因在原发性胆囊癌（PGC）中的表达及其意义。［ 方法 ］ 应用 原位杂交（DNA-RNA）技术与免疫组化SP法分别检测64例PGC中PTEN mRNA、PTEN蛋白和c-erbB-2蛋白的表达。以22例胆囊腺瘤及10例慢性胆囊炎作为对照。［结果］PTEN mRNA和蛋白在PGC组织中的阳性表达率(56.3%，59.4%)均明显低于胆囊腺瘤组织（81.8%，90.8%）和慢性胆囊炎组织(100%，100%)(P＜0.05)。c-erbB-2蛋白在PGC组织中的阳性表达率(46.8%)均明显高于胆囊腺瘤组织(9.1%)和慢性胆囊炎组织(0)（P＜0.05）。［结论］ PTEN低表达和c-erbB-2高表达共同参与了PGC的发生 发展 ，其蛋白可作为判断PGC预后的指标。 【关键词】 胆囊肿瘤 Expression of PTEN and c-erbB-2 in 64 Cases with Primary Gallbladder Carcinoma HUANG Ying1, WAN Hui-bin2, ZHENG Chang-li3 （1. The Primary Department of Medical in Changde Vacational Technical College, Changde 415000, China; 2.The Hospital of Womens Prison in Chang-BEi, Nanchang 330038, China; 3.The Pathology Department in Xiangya Hospital of Centre-South University, Changsha 410000, China) Abstract: ［Purpose］ To study the expression of PTEN and c-erbB-2 in primary gallbladder carcinoma(PGC) and its significance. ［Methods］ The expression of PTEN mRNA, PTEN protEIn and c-erbB-2 protein were detected by in situ hybridization(DNA-RNA) and immunochemistry(SP method) in 64 cases with PGC, 22 cases with gallbladder adenoma and 10 cases with chronic cholecystitis as controls. ［Results］ The positive expression rates of PTEN mRNA and PTEN protein in PGC(56.3%,59.4%) were significantly lower than those in gallbladder adenoma (81.8%,90.8%) and chronic cholecystitis(100%,100%)(P＜0.05）. The positive expression rate of c-erbB-2 protein in PGC(46.8%) was significantly higher than that in gallbladder adenoma (9.1%) and chronic cholecystitis (0)(P＜0.05）. ［Conclusion］ Low expression of PTEN and high expression of c-erbB-2 both play an important role in oncogenesis of PGC. They may be uses as markers for prognosis evaluation. Key words: gallbladder neoplams；c-erbB-2；PTEN；immunochemistry；in situ hybridization 近年来,与原发性胆囊癌（primary gallbladder carcinoma，PGC）发生有关的癌基因、抑癌基因不断被发现[1]。抑癌基因-10号染色体上缺失的磷酸酶及张力蛋白同源物基因（phosphatase and tensin homology deleted on chromosome ten，PTEN），于1997年由Li等[2