TNF-α基因单核苷酸多态性与HBV感染结局_0.docVIP

TNF-α基因单核苷酸多态性与HBV感染结局 【关键词】 ,乙型肝炎；TNF-α基因；单核苷酸多态性（SNPs）；单体型 　　摘要： 目的 探讨肿瘤坏死因子α（TNFα）基因启动子区238G/A、-857T/C、-863C/A与乙型肝炎病毒（HBV）感染结局之间的关系。 方法 采用病例-对照 研究 方法，募集244例HBV自限性感染者、212例HBsAg携带者和391例慢性乙型肝炎患者作为研究对象， 应用 聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对（TNF-α）基因启动子区-238G/A、-857C/T、-863C/A基因型进行测定。结果 自限性感染者携带-238A等位基因的频率显著低于HBsAg携带者（P=0.04）和慢性乙肝患者（P=0.047）；慢性乙肝患者携带TNF-α-857C等位基因的频率显著高于HBsAg携带者（P=0.0008）和自限性感染者（P=0.03）；慢性乙肝者TNF-α-863A等位基因频率显著高于HBsAg携带者（P=0.02）。应用多元Logistic回归 分析 ，控制年龄、性别等混杂因素后，慢性乙肝患者与HBV自限性感染者比较，TNF-α-857CC和TNF-α-238 GA与慢性乙肝显著关联（OR=1.53,P=0.044;OR=2.11，P=0.045）；慢性乙肝患者与HBsAg携带者比较，TNF-α-857CC与慢性乙肝显著关联（OR=1.92,P=0.004）；HBsAg携带者与HBV自限性感染者比较，TNF-α-238GA与HBsAg携带者显著关联（OR=2.34,P=0.020）。结论 TNF-α基因启动子区多态性可能是 影响 HBV感染结局的重要危险因素。 　　关键词： 乙型肝炎；TNF-α基因；单核苷酸多态性（SNPs）；单体型 　　Relationship between polymorphisms of TNF-α gene promoter region and HBV infection outcome 　　Abstract： Objective To determine when ther -238G/A,-857T/C and -863C/A polymorphisms of tumor necrosis factor-alphya (TNF-α) gene promoter were associated with outcomes of hepatitis B virus (HBV) infection.Methods A total of 244 HBV self-limited infected subjects,212 asymptomatic HBsAg carriers (HBsAg carriers) and 391 chronic hepatitis B (HB) patients were recruited to conduct a case-control study.TNF-α-238G/A,-857C/T and -863C/A gene promoter polymorphisms were examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Results The frequency of -238 allele A in self-limited individuals was 2.6%,significantly lower than 5.0% in chronic HB patients and 5.3% in HBsAg carriers (P=0.04 and P=0.047),respectively.The frequency of TNF-α-857 allele C in chronic HB patients was 87.5%,significantly higher than 80.5% in HBsAg carriers and 82.8% in self-limited individuals (P=0.0008 and P=0.03),respectively.The frequency of TNF-α-863 allele A in chronic HB group was 77.5%,significantly higher than 71.5% in HBsAg carriers (P=0.02).Multiple logistic regression analyses indicated an increased risk of chron