2004-梨内源与外源核糖核酸酶对花粉萌发及花粉管生长的影响.pdf

中国农业科学 2004,37（6）：891-895 Scientia Agricultura Sinica 梨内源与外源核糖核酸酶对花粉萌发及花粉管生长的影响 张绍铃，徐义流，陈迪新，徐国华,赵彩平 （南京农业大学园艺学院，南京 210095） 摘要：将从沙梨 （Pyrus pyrifolia）花柱中分离出的RNase（内源RNase）和外源RNase添加于花粉萌发培 养基培养二十世纪梨花粉。结果表明，低活性量（0.006 U）的RNase对花粉萌发及花粉管生长的抑制作用弱；较 高活性量（0.010U）的RNase T对花粉萌发及花粉管生长的抑制作用最强，其次为二十世纪花柱S-RNase和RNase 1 A；新水花柱S-RNase对花粉管生长抑制作用最弱；在添加这4种RNase的培养基中，二十世纪花粉管生长的长度分 别是对照花粉管长度 （410µm）的37%、40%、77%和79%。不含S-RNase的花柱RNase虽然活性较高，但对自花花 粉的萌发及花粉管生长没有抑制作用。 关键词：梨；内源RNase；外源RNase；花粉萌发；花粉管生长 Effects of Stylar RNase and Exogenous RNase on Pollen Germination and Tube Growth in Japanese Pear (Pyrus pyrifolia Nakai) ZHANG Shao-ling, XU Yi-liu, CHEN Di-xin, XU Guo-hua, ZHAO Cai-ping (College of H orticulture, Nanj ing Agricultural University, N anj ing 210095) Abstract: Th e results of Nij isseik i (Pyrus py rif olia) pollen cu ltivation in vitro by addin g stylar RNase or exogen ous RNase to the basal medium showed that when the activity of RNase was relatively weak (0.006 U), the inhibitory effect on pollen germination and tube growth was also relatively weak . When the activity of RNase was relatively strong (0.0 10 U), the inhibitory effect of RNase T1 on pollen germination and tube growth was th e strongest, followed by Nij isseiki stylar S- RNase and RNase A, and Sh insui stylar S-RN ase showed the weakest inhibitory effect on pollen tube growth . Th e lengths of Nij isseiki pollen tubes cultivated by addin g RNaseT , N ij isseiki stylar S-RNase, RNase A and Shinsui stylar S-RN ase 1 separately to their media were 37%, 40%, 77% and 79% of the control length which was (4 10 µm), respectively . Although stylar RNase without S-RNase had intensified RNase activity, it did not have any effect on self-pollen germination and tube gr