工程大肠杆菌脂肪酸胞外分泌调控研究进展_孟鑫.pdf

※专题论述 食品科学 2011, Vol. 32, No. 05 331 工程大肠杆菌脂肪酸胞外分泌调控研究进展 1 1,2 1 1 1 1 1, 孟 鑫 ，程 涛 ，李良智 ，杨建明 ，刘 炜 ，徐 鑫 ，咸 漠 * ( 1.中国科学院青岛生物能源与过程研究所，山东青岛 266 10 1； 2.江西理工大学材料与化学工程学院，江西赣州 34 1000) 摘 要：天然产油微生物产生的脂肪酸多以甘油三酯形式贮存在细胞内，需要从细胞内提取，工艺复杂、生产成 本高，且严重污染环境。工程大肠杆菌定向调控生产游离脂肪酸已成为近年来的研究热点，是缓解能源危机，提 供优质、低成本油脂原料的有效途径。本文从大肠杆菌脂肪酸胞外分泌相关基因(tesA 、f adD 、f adL ) 和调控蛋白 (MsbA)两个方面综述脂肪酸胞外分泌调控研究进展，并提出了利用分子生物学和代谢工程手段对大肠杆菌脂肪酸代 谢定向调控，生产胞外游离脂肪酸的新的研究方向。 关键词：脂肪酸；代谢调控；转运 Research Progress in Regulation of Fatty Acid Secretion in Engineered E. coli Strain 1 1,2 1 1 1 1 1, MEN G X in ，CHEN G Tao ，LI Lian g-zhi ，YAN G Jian -ming ，LIU Wei ，XU X in ，XIAN Mo * (1. Qingdao In stitute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266 10 1, China ； 2. School of Materials and Chemical Engineering, Jiangxi University of Science and Technology, Ganzhou 34 1000, China) Abstract ：Fatty acids produced by oil-producing microorganisms are stored in cells as the form of triglyceride. The extraction of these fatty acids from the cells is involved in complex processing, high production cost and serious environmental pollution. Targeted regulation for the production of free fatty acids by engineered E. coli strains is becoming one of the hottest topics in current research , which will be an effective strategy for reducing energy crisis and providing high-quality and low-cost raw materials of oil. The research progress of some genes (tesA ,f adD andf adL) and regulatory protein (MsbA) related to extracellular secretion of fatty acids in E. coli has been reviewed in t