脑溢安颗粒剂对出血性中风大鼠缺血_省略_HSP70与NF_kB表达的影响_曾锦旗.pdf

2002 8 6 20 ( 总第420 页) Chinese J urnal f Basic Medicine in Traditi nal Chinese Medicine 20021V l. 8. N . 6 = X HSP70 NF-kB 曾锦旗, 黎杏群, 金益强 ( 中南大学湘雅医院中西结合研究所, 湖南长沙 410008) : 目的: 研究脑溢安( NYA) 颗粒剂对出血性中风大鼠脑内HSP70 与NF-kB 表达的影响 方法: 用× 型胶原酶 体定位法复制出血性中风动物模型, 观察行为TUNEL 及HE 染色, HSP70 NF-kB 免疫组化反应, 取手术侧海马及皮质分别抽提总蛋白与核蛋白进行Western bl t 反应, 密度扫 描测定HSP70 与NF-kB 蛋白表达结果:NYA 治疗组爬行计分明显低于模型对照组, 3d 时血肿明 显缩小, 7d 基本吸收, 凋亡细胞明显减小; 免疫组化示3d 时可见,HSP70 信号增强, NF-kB 信号相对 减弱; Western bl t 及密度扫描显示HSP70 显著升高,NF-kB 显著降低结论: NYA 能促进神经功能 恢复, 加快血肿吸收; 增强HSP70 表达, 抑制NF-kB 表达, 通过抑制炎症因子保护脑出血后继发脑 缺血损伤可能是NYA 的作用机理之一 : 脑溢安颗粒剂; 出血性中风; 大鼠; 免疫组化; Western bl t;HSP70; NF-kB : R28515 : B : 1006-3250( 2002) 06-0020-04 Effects of Traditional Chinese Medicine Nao-Y-l An Granule on the E pression of HSP70 and NF-kB and Apoptosis in Ischemia Corte of Rat Brain after E perimental Haemorrhagic Stroke ZENG Jin-qi,LI Xing-qun, JIN Y-i qiang ( Institute of Combined Traditional and Western Medicine fo Xiang YaHospital Center-Southern University, Changsha 410008, China) Abstract: Objective:T bserve the effects f Traditi nal Chinese Medicine Na-Y-i An Granule n the expressi n f HSP70 and NF-kB in rat brain after experimental haem rrhagic str ke. Meth ds: Haem rrhagic str ke rat m del was made by injecting c llagenase type × stere taxically, and was taken f r Behavi r bservati n; TUNEL and HE staining, HSP70 and NF-kB immun hist chemistry; c rtex f perati n side were taken, then t tal pr tein and nucl