实验二 绿豆芽的蛋白质含量测定(国外英文资料).docVIP

实验二 绿豆芽的蛋白质含量测定(国外英文资料) Lung zhimin 20093022012109 pharmaceutical class Test the protein content of green bean sprouts (cowa bright blue g-250 colorimetric method) One, the purpose of the experiment Understand the principle of testing the protein content of the cooma brilliant blue method, and master its measuring method. Master the use of spectrophotometer. Second, the experimental principle Coomassie brilliant blue G - 250 is a kind of dye, under free state in red, when it is combined with protein into blue, after the combination with maximum light absorption under 595 nm wavelength. In a certain protein concentration range (0 ~ 1000 mu g/mL), its absorbance is proportional to the protein content, so it can be used for quantitative determination of soluble protein. Instruments, reagents and materials 1, the instruments and equipment Analytical balance, 10mL plastic centrifugal tube, 5mL pipette, mortar, measuring cylinder, 50mL centrifuge, 10mL volumetric flask, centrifuge, 722 spectrophotometer 2, reagents (1) the standard protein original liquid: called the 100mg of serum albumin, dissolved in distilled water and fixed to 100mL, the standard protein source for 1mg/mL. (2) caumarssparkly blue g-250 reagent: it is called 100mg of macasbright blue g-250, soluble in 50mL 95% of ethanol, added 85% (m/v) phosphatic 100mL, and then in distilled water to 1000mL. This solution can be placed at room temperature for one month. Ingredients: mung bean sprouts Four, the operation step (1) the preparation of green bean sprouts protein samples 1, accurately say fresh mungbean sprout, hypocotyl 2 g in the mortar, add 2 ml of distilled water research into homogenate, transferred to the 50 ml centrifuge tube, with 6 ml distilled water washing mortar, washing liquid to collect in the same measuring cylinder, the capacity to 10 ml, blending; Place 10 to 20 min in an ice bath for maximum withdrawal. Take 1ml to centrifuge tube from the measuring cylinder, 12000rpm centrifuge 5min, p