小鼠骨髓中嗜多染红细胞(PCE)微核试验简介(国外英文资料).docVIP

小鼠骨髓中嗜多染红细胞(PCE)微核实验简介(国外英文资料) A brief introduction of the PCE micronuclear test in mouse bone marrow Micronucleus (micronucleus), related to chromosome damage, is no chromosome or chromatid centromere fragment or spindle fiber damage and lost the whole chromosome, legacy in the late of cell division in the cytoplasm, after the end of the separate forms the rule of one or a few times nuclear, included in the cytoplasm of the daughter cells, is smaller than the main nuclear, therefore calls the microkernel. So micronuclear tests can detect changes in chromosomal integrity and chromosome separation induced by chemical or physical factors to alter the end of the two genes. The experiment purpose Study and master the method of micronuclear determination of the bone marrow polycyte (PCE) in mice To understand the cell chromosome damage, the determination of the faulting agent and some of the non-euploid agents Further proficient in production and examination Equipment and reagents 1. The equipment Surgery cut, toothless forceps, small bending hemostatic forceps, slides, glass dye VAT, clock, drying rack, microscope lens (with oil), syringes and needles, clean cotton gauze, nipple straws, glass pencil, electric hair dryer 2. The reagent Methanol, Giemsa reserve fluid, phosphate buffer (PH6.8) cyclophosphamide steps Experiment with animals and treatment (1) animal choice: the most commonly used mouse, 18-20g, each group of 10, is male and female. (2) infected way: according to the research purpose or physical properties of different subjects, in principle, can adopt the way of human touch as far as possible, usually by lavage and intraperitoneal injection. (3) the number of times the virus is infected: a number of detoxification (a daily infection, four days in a row, a fifth day of sampling) or two methods of poisoning (before the death of 30h + death) (4) the dose and control selection were selected for the LD50, with a maximum dose of 1/2LD50, and 3 to 4 doses. And the