异常白细胞检查(国外英文资料).docVIP

异常白细胞检查(国外英文资料)The ABCD peoples hospital for examination room work instructionFile number: ABCD - 3-lj-43Revision/revision number: A / 0Topic contentAbnormal leukocyte examinationEffective date:age 4:5 pagesAbnormal leukocyte examination (microscopy)Experimental principleWith ritz dyeing, the preparation of blood smear was stained and then morphologically examined under the microscope.Sample collection:Type 1 specimen: newly extracted anticoagulant or fingertip blood.2.2 sample requirements:2.2.1 anticoagulants are anticoagulant with EDTA K2.2.2.2 using the tip of the finger to test for white blood cells, such as the finger with chilblains, the idea is to use earlobe blood.The specimen is stored: it should be immediately sent to the blood smear and sent to check as soon as possible.Transportation: keep dry, at room temperature.Specimen rejection standard: contamination, coagulation specimen cannot be determined.Experimental materials:The dyeing liquid of reyes blood cells, the manufacturer: (taiwanese-owned) zhuhai beso biotechnology co., LTD.Laboratory instruments:Instrument name: OLYMPUS microscopeInstrument manufacturer: OLYMPUS7.3 instrument model: OLYMPUS CH30Step 8The blood membrane should be tongue shaped, head, body, and tail.Ruis dyeing 8.2: puts system good dry blood smear staining the rack, jarretts dyeing drops A 3-5 drops to make it fast blood covered with film, then add rays dyeing liquid B 3-5 drops, rocking gently slides or use A washing ear ball to blow gas to make A and B dye solution blending adequately, dyeing 1 to 2 minutes (low temperature or smear thicker can be appropriately extended dyeing time), use water to dye solution, stay dry.High magnification (oil mirror) is observed in the form of white blood cells.Outcome judgment and analysisThe 9.1 nuclear image changes.Nuclear left: (1) shows that naive or rod nuclei in the peripheral blood granulocyte increased, can be found in acute leukemia, acute pyogenic bacteria infection, acute