大鼠白细胞介素-6(IL-6)说明书(国外英文资料).docVIP

大鼠白细胞介素-6(IL-6)说明书(国外英文资料) The rat leucocyte di-6 (il-6) enzyme-linked immunoassay Use the instruction manual for the kit This test kit is for study only. Detection range: 96 t 3 pg/ml - 120 pg/ml Purpose: The experimental kit was used to determine the content of interleukin-6 (il-6) in serum, plasma and related fluids in rats. The experimental principle The experimental formulae were used to determine the level of interleukin-6 (il-6) in the specimen. Rats with purified interleukin 6 (IL - 6) antibody package is microporous plate, made from solid phase antibody, the package in the sheet resistance of the microporous, in turn, to join the interleukin 6 (IL - 6), and then with HRP markers interleukin - 6 (IL - 6) antibodies, antibody antigen - enzyme mark antibody complex formation, thoroughly with the substrate TMB color after washing. TMB is converted into blue by the catalysis of HRP, and is converted to the final yellow by acid. The depth of the color was positively associated with the interleukin-6 (il-6) in the sample. The absorbance (OD) was determined at 450nm wavelength by the enzyme marker, and the concentration of interleukin-6 (il-6) in the sample was calculated by the standard curve. Kit of one 30 times concentrated detergent 20 ml x 1 bottle 7 Termination of the liquid 6 ml x 1 bottle 2 Enzyme standard reagent 6 ml x 1 bottle 8 Standard (240pg/ml) 0.5 ml x 1 bottle 3 Enzyme standard package is plate Article 12 hole x 8 9 Standard diluent 1.5 ml x 1 bottle 4 The sample diluent 6 ml x 1 bottle 10 The instructions 1 5 A liquid agent 6 ml x 1 bottle 11 Sealing plate membrane 2 6 Chromogenic agent B liquid 6 ml x 1 / bottle 12 bags 1 Samples required The specimen can be extracted as early as possible, and the extraction is carried out according to relevant literature, and the experiment should be carried out as soon as possible. If not immediately, can put in the specimen - 20 ℃ to save, but should avoid repeated freezing and thawing Cant detect NaN3 sample