细胞生物学实验考考试样题及实验思考题答案(国外英文资料).docVIP

细胞生物学实验考考试样题及实验思考题答案(国外英文资料) Test of cell biology experiment 1, fill in the blanks (2 minutes/time, 30 points) The lotion is made up of dichromate, concentrated sulfuric acid and distilled water. 2, 1640 medium includes RPMI1640 medium, glutamate, double reactance, calf serum. 3, the dye that identifies living cell is 0.4 hope blue solution; The dye that identifies the nucleus of the nucleus is methyl green - parroin dye; Identify the line The dye of grain is neutral red - jennas green dye. The basic step of the separation of the nucleus is to organize the slurry, centrifuge, purification and identification. The dye that makes proteins preserved in the cell skeleton is 1 percent Triton - 100. Second, short answer (5 + 5 + 10) 1. The mitochondria in the process of extraction, why in 0 ~ 4 ℃? Answer: in order to maintain the physiological activity of the component; The enzyme in the mitochondria is destroyed and the enzyme is not lost What are we going to do with a certain concentration of reagents when were detecting cell permeability? A: to make sure that the experiment is carried out under the condition of seepage; To prevent the entry and exit of substances; It also avoids the effects of osmotic pressure. Count steps and methods of cells. Answer: the dye that adds the volume of the volume and the cell hang fluid to mix ~ ~ put the droplet on the counting board to bottom double mirror to observe, the living cell colorless, dead The cell is blue, and the total number of cells in four squares is counted. (to the big grid, to the left, not to the right.) the calculation. Ⅰ: per milliliter concentrate cells number = 4 big / 4 * 100 * total dilution multiple Ⅱ: the cell survival rate (%) = (the total number of cells - the number of dead cells)/total number of cells. The cell biology experiment is the answer The experiment USES various microscopes to observe cell morphology and structure Briefly describe the main structure and operation of the microscope. The general bi