益气养阴活血法对糖尿病大鼠额叶皮质神经元NT-3表达的影响.docVIP

益气养阴活血法对糖尿病大鼠额叶皮质神经元NT-3表达的影响 作者：陈伟燕，张跃明，葛刚锋，刘岩 【摘要】 目的 探讨益气养阴活血法对糖尿病大鼠额叶皮质神经元神经营养因子3（NT-3）表达的 影响 。 方法 用链脲佐菌素（STZ）腹腔注射诱发糖尿病大鼠模型，益气养阴活血药灌胃给药进行防治，8周后取脑做NT-3免疫组化染色。结果 正常对照组大鼠额叶皮质内NT-3阳性神经元数目多，染色深；糖尿病模型组大鼠额叶皮质内NT-3阳性神经元数目少，染色浅；益气养阴活血法 治疗 组大鼠数目和染色与正常组大鼠接近。结论 益气养阴活血法具有防治糖尿病大鼠额叶皮质神经元退行性变的功效。 【关键词】 糖尿病 益气养阴活血法 额叶 神经营养因子3 大鼠 Effects of Yiqi Yangyin Huoxue principle on the expression of neurotrophin-3 of the frontal lobe cortex neurons in diabetic rat brain 【Abstract】 Objective To explore the effects of Yiqi Yangyin Huoxue principle on the expression of neurotrophin-3 of the frontal lobe cortex neurons in diabetic rat brain.Methods The diabetic rat model was produced by intraperitoneal injection of streptozotocin(STZ).The Yiqi Yangyin Huoxue drugs was poured into the stomach of the treated group. The brains were taken and the expression of neurotrophin-3 were estimated with immunochemistry 8 weeks later.Results Immunochemistry showed that there were many positive cells in the frontal lobe cortex of the normal group and they were deeply dyed.Positive cells of the model group decreased and thEir color was shallow .The number and the color of positive cells in the treated group were close to that of the normal group.Conclusion The Yiqi Yangyin Huoxue principle can effectively improve the degeneration of the frontal lobe cortex neurons in diabetic rat brain. 【Key words】 diabetes Yiqi Yangyin Huoxue method frontal lobe neurotrophin-3 rat 糖尿病属中医消渴症的范畴，是一种严重危害人体健康的常见病、多发病。糖尿病已成为继肿瘤、心血管疾病之后的第三大严重的非传染性疾病，糖尿病晚期病人常伴脑神经元的退行性变。神经营养因子3（NT-3）广泛分布在中枢神经系统内，具有维持神经元存活、诱导其轴突生长和促进损伤神经元的修复及阻止神经元损伤后死亡的功能。本实验观察养阴活血法治疗后糖尿病大鼠额叶皮质神经元NT-3的表达的改变，探讨此中医药疗法能否防止糖尿病大鼠额叶皮质神经元的退变。 1 材料与方法 1.1 试验药物及试剂 益气养阴活血中药复方主要由黄芪30g，淮山药15g，葛根15g，枸杞子15g，丹参15g，红花12g，麦冬15g，马齿苋15g等组成，组成药物均购自浙江省中 医院 中药房，由浙江中医学院制剂室制备成含生药4g/ml，4℃冰箱保存备用。链脲佐菌素（STZ）和免疫组化试剂盒均为Sigma公司产品。 1.2 动物分组及模型复制 健康雄性SD大鼠30只，清洁级，体重：200～250g ，购自浙江大学医学院动物实验中心。适应性饲养1周后，将大鼠随机分三组：正常对照组、糖尿病模型组和益气养阴活血法治疗组，每组各10只，后两组大鼠空腹12h后腹腔注射pH4.4枸橼酸钠缓冲液配成的1.25% STZ，按50mg/k