基因聚合对水稻稻瘟病的抗性影响.pdfVIP

分子植物育种 ,2003 年 ,第 1 卷 ,第 1 期 ,第 22 —26 页 Molecular Plant Breeding ,2003 ,Vol1 ,No1 ,22 —26 　 研究报告 RESEARCH REPORT 基因聚合对水稻稻瘟病的抗性影响 刘士平 　李 信 　汪朝阳　李香花 　何予卿 华中农业大学作物遗传改良国家重点试验室 , 武汉 , 430070 通讯作者 , yqhe @mail. hzau. edu. cn 摘要 水稻稻瘟病是世界上广泛发生的水稻真菌性病害之一 。本研究利用 100 个稻瘟病菌株对以 CO39 为 背景且带有单个基因和多个基因聚合的近等基因系进行接种分析 , 结果表明 , Pi 1 和 Pi 2 属两个广谱高 抗稻瘟病基因 , 两基因可在我国南方稻区加以合理利用 。基因聚合后抗谱增宽、抗性加强 , 说明基因聚 合是培育稻瘟病持久抗性品种的有效方法 。 关键词 近等基因系 , 基因聚合 , 稻瘟病 , 水稻 Gene Pyramiding to Increase the Blast Resistance in Rice Liu Shiping 　Li Xin 　Wang Zhaoyang 　Li Xianghua 　He Yuqing National Key Laboratory of Crop Genetic Improvement , Huazhong Agricultural University , Wuhan , 430070 Corresponding author , yqhe @mail. hzau. edu. cn ABSTRACT Rice blast is one of the most widespread and destructive fungal diseases worldwide. In this study , 100 rice blast monoconidial isolates were used to inoculate a set of CO39 nearisogenic lines with either single or p yra miding genes for evaluating the interaction between the different resistant genes and the patho gen. The results showed that Pi 1 and Pi 2 are widespectrum genes. Lines with any of these two genes showed resistant to most of the Chinese fungal isolates. Lines with more resistant genes had higher resistant levels and resistant to more isolates. This implies that gene p yramiding can increase the spectrum and the strength of resistance to fungi blast. It can be concluded that gene p yramiding is an effective approach for the improvement of rice varieties with durable resistance to blast . KEYWORDS Near iso genic lines , Gene p yramiding , Pyricularia grisea , Rice 基本聚合对水稻稻瘟病的抗性影响