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昆仑雪菊挥发油化学成分及对新生隐球菌抗菌作用
Jun. 20, 2016, 43(6): 1304−1314
Microbiology China /wswxtbcn
tongbao@ DOI: 10.13344/j.microbiol.china.150500
*
(
843300)
GC-MS
(MIC)
0.781 µL/mL
Essential oil composition of Coreopsis tinctoria Nutt. and
anti-Cryptococcus activity
*
ZHANG Yan-Mei FENG Zi-Kai ZENG Hong
(Xinjiang Production Construction Corps, Key Laboratory of Protection and Utilization of Biological Resources in
Tarim Basin, College of Life Sciences, Tarim University, Alar, Xinjiang 843300, China)
Abstract: [Objective] Essential oil (EO) composition of Coreopsis tinctoria Nutt., together with The
their anti-Cryptococcus activity and influence on the cell membrane of Cryptococcus neoformans
present study were evaluated in this study. [Methods] EO from Coreopsis tinctoria Nutt. was extracted
team distillation and the main chemical components of it was identified by GC-MS. The micro-dilution
method was used in acquiring the minimal inhibitory concentration (MIC). The cell weight of C.
neoformans and germ tube germination of C. neoformans were also determined to evaluate the anti-C.
neoformans activity in vitro. The effect on membrane permeabilization was measured by monitoring
the influx of Propidium iod
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