冬虫夏草菌丝体水提醇沉物体外抗肿瘤活性研究.PDF

- 108 - Drug Evaluation Research 第 37 卷 第 2 期 2014 年 4 月 冬虫夏草菌丝体水提醇沉物体外抗肿瘤活性研究 1, 3 1, 3 1, 2* 1, 3 1, 3* 陈家念 ，张 璇 ，蔡豪斌 ，傅晓波 ，沈星灿 1. 广西师范大学 药用资源化学与药物分子工程教育部重点实验室，广西 桂林 541004 2. 桂林英美特生物技术研究所，广西 桂林 541001 3. 广西师范大学 化学与药学学院，广西 桂林 541004 摘 要：目的 研究冬虫夏草菌丝体水提醇沉物的体外抗肿瘤活性及其机制。方法 采用热水浸提-醇沉法得到水提物，高 效液相色谱仪测定水提醇沉物中腺苷的量；采用 MTT 法测定其抗肿瘤活性，利用流式细胞仪结合碘化丙锭染色法检测其对 细胞周期的抑制。结果 实验表明水提醇沉物能抑制人肝癌 HepG2 细胞株及大细胞肺癌 NCI-H460 细胞株的增殖，并呈浓 度相关性；半数抑制浓度（IC50 ）值分别为（1.49±0.19 ）和（1.67±0.27 ）mg/mL 。细胞周期分析表明，水提醇沉物分别阻 滞 HepG2 及 NCI-H460 细胞周期于 G2/M 期、S 期，并可诱导上述两种细胞发生凋亡。结论 冬虫夏草水提醇沉物通过阻滞 HepG2 及 NCI-H460 细胞周期循环，诱导其凋亡，从而表现出良好的增殖抑制活性。为深入研究冬虫夏草菌丝体水提醇沉物 抗肿瘤的机制提供了实验证据。 关键词：冬虫夏草菌丝体；水提物；抗肿瘤；机制 中图分类号：R285.5 文献标志码：A 文章编号：1674 - 6376 (2014) 02 - 0108 - 05 DOI: 10.7501/j.issn.1674-6376.2014.02.003 Anti-tumor activity of water extracts from Cordyceps sinensis mycelia in vitro CHEN Jia-nian1, 3, ZHANG Xuan1, 3, CAI Hao-bin1, 2, FU Xiao-bo1, 3, SHEN Xing-can1, 3 1. The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), Guangxi Normal University, Guilin 541004, China 2. Institute of Guilin Immunetech, Guilin 541001, China 3. School of Chemistry and Pharmacy, Guangxi Normal University, Guilin 541004, China Abstract: Objective To study the anti-tumor activity of water extract from artificially propagated Cordyceps sinensis mycelia in vitro and its mechanisms. Methods Water extract from C. sinensis mycelia was obtained using hot-water-extraction combined with alcohol precipitation method and the content of adenosine was determined using HPLC; MTT assay was performed to assess the cell growth inhibition, and the distribution of cell cycle was determined by flow cytometric analysis of propidium iodide staining. Results It was found that the water extract of C. sinensis myce