小肠脂酸联合蛋白基因多态性与2型糖尿病回并冠芥蒂的关....docVIP

小肠脂酸结合蛋白基因多态性与2型糖尿病合并冠心病的关... 小肠脂酸结合蛋白基因多态性与2型糖尿病合并冠心病的关系 2011-01-16 黄敏　杨香玖　李丽　胡正国　王中丽 　　【摘要】　目的　为了解小肠脂酸结合蛋白基因(FABP2)多态性与2型糖尿病合并冠心病(CHD)的关系。方法　2型糖尿病无冠心病组(DM-1)64例，2型糖尿病合并冠心病组(DM-2)82例，非糖尿病冠心病组62例，正常对照组60例。应用聚合酶链反应(PCR)技术检测268例对象FABP2 Hha I位点的限制性片段长度多态性(RFLPs)。结果　(1)武汉地区汉族人存在PABP2 Hha I多态性位点，可产生Thr54(-)和Thr54(+)多态片段。(2)2型糖尿病合并冠心病患者FABP2 Thr54(+)基因型频率显著高于正常人(P＜0.05)。(3)FABP2 Thr54(+)基因类型对2型糖尿病合并冠心病的参与风险率为25.6%，比数比为2.13(95% 可信限是1.05～4.45)。(4)2型糖尿病合并冠心病组含FABP2 Thr54(+)基因类型患者与含FABP2 Thr54(-)基因类型患者比较，空腹胰岛素水平显著增高(P＜0.01)，胰岛素敏感指数显著下降(P＜0.05)。(5)2型糖尿病合并冠心病组含FABP2 Thr54(+)基因类型患者的血甘油三酯水平显著高于含FABP2 Thr54(-)基因类型患者(P＜0.05)，而高密度脂蛋白胆固醇水平显著低于含FABP2 Thr54(-)基因类型患者(P＜0.05)。结论　(1)武汉地区汉族人存在FABP2多态性。(2)FABP2多态性可能与2型糖尿病合并冠心病有一定关联。(3)FABP2多态性可能与2型糖尿病合并冠心病的胰岛素抵抗相关。(4)FABP2多态性可能是2型糖尿病合并冠心病脂质代谢异常的影响因素之一。 　　【关键词】　小肠脂酸结合蛋白　基因　限制性片段长度多态性　糖尿病，2型　冠心病 Relationship between polymorphism of the human intestinal fatty acid binding protein gene and type 2 diabetic patients with coronary heart disease HUANG Min,YANG Xiangjiu,LI Li,et al Department of Endocrinology,The Second Affiliated Hospital of Hubei Medical University,Wuhan,430071 　　【Abstract】　Objective　To study the relationship between the human intestinal fatty acid binding protein gene (FABP2) and type 2 diabetic patients with coronary heart disease. Methods　The polymorphism of 54th codon of FABP2 was determined with the PCR and RFLP methods in 268 subjects, inclnding 82 cases of type 2 diabetes with coronary heart disease (CHD), 64?cases of type 2 diabetes without CHD, 62 cases of non-diabetic CHD and 60 normal controls. Results　The polymorphic restri?tion site has been reported in the FABP2 identified by the enzyme Hha I in Han nationality in Wuhan. The frequency of genot?pe Thr54(+) significantly i?creased in the type 2 diabetic patients with coronary heart disease as compared with that in healthy subjects (P＜0.05). There was no significant difference in the frequencies among coronary heart disease, type 2 diabetes, type 2 diabetic pati