水稻第1染色体 qTGW1．2区域粒重组分性状 QTL 的剖析.pdf

中国水稻科学 (ChinJRiceSci)，2015，29(3)：232—240 httpt w ．ricesci．cn 232 DOI：10．3969／j．issn．1001—7216．2015．03．002 水稻第 1染色体 qTGW1．2区域粒重组分性状 QTL的剖析 王琳琳 陈玉宇 郭梁 张宏伟 樊叶杨 庄杰云 (中国水稻研究所 国家水稻改 良中心／水稻生物学国家重点实验室，杭州 310006； 通讯联系人 ，E—mail：zhuangjieyun@caas．cn) DissectionofQuantitativeTraitLociforGrain W eightand ItsComponent TraitsintheqTGW 1．2 RegiononChromosome1ofRice WANGLin-lin，CHENYu—yu。GuoLiang，ZHANGHong-wei，FANYe—yang，ZHUANGJie—yun (ChineseNationalCenterforRiceJ r0P7P￡／S￡n KeyLaboratoryofRiceBiology，ChinaNationalRiceResearch Institute， Hangzhou310006，China； Correspondingauthor，E-mail：,~huangJieyun@caas．cn) W ANG Linlin，CHEN Yuyu，GUO Liang，eta1．Dissectionofquantitativetrait1ociforgrainweightanditscomponent traitsintheqTGW1．2regiononchromosomelofrice．ChinJRiceSci，2015，29(3)：232-240． Abstract：Thispaperreportsthedissectionofthreequantitativetraitloci(QTLs)forlO00-grainweight(TGW)，grain length(GI)andgrainwidth (GW )onthelongarm ofchromosomelinrice．A plantcarryingaheterozygoussegment extendingfrom RM212toRM265wasselectedfrom theZhenshan97。／Milyang46BC2F8population．Theresulting BC2F9populationwasassayedwithDNA markers．Twoplantswereidentified，carryingheterozygoussegmentscovering theintervalsRM212一 RM 11787 and RM 11787一 RM265 which werepreviouslyfound to harborqTGW 1．2n and qTGW 1．26forTGW ，respectively．TwoBC2F10populationswereproduced，from which threeplantswithsequential heterozygousregionswereidentified，respectively．UsingthesixBC2F1lpopulationsderived，aQTLforGLwasdetected intheinterva1RM 1173O— RM 11800，andtheregionsforqTGW 1．2口andqTGW 1．26werenarroweddown tothe58O kb intervalRM 11730一W n33304and2．0Mb intervalRM 11807一 RM 1l885，respectively．Inthemeantime，fiveplants withsmallerheterozygousregionswereselecte