重要---ANG系统在糖尿病视网膜的作用.pdfVIP

The Angiopoietin/Tie-2 System Regulates Pericyte Survival and Recruitment in Diabetic Retinopathy Jun Cai, 1 Oksana Kehoe,2 Gill M. Smith,2 Philip Hykin,3 and Michael E. Boulton1 PURPOSE. The angiopoietin (Ang) system plays an important anism is not well understood. In addition, the maturation of role in vascular stabilization and pathologic neovascularization. newly formed vessels during the late stages of proliferative The hypothesis for the study was that, in addition to modulat- diabetic retinopathy requires the incorporation of pericytes.4 ing endothelial cell behavior, the angiopoietin/Tie-2 system The angiopoietins, a recently discovered family of vascular also regulates the pericyte apoptosis and/or the vessel matura- regulatory molecules binding to the Tie-2 tyrosine receptor, tion associated with diabetic retinopathy. play an important role in retinal neovascularization.5,6 The METHODS. Tie-2 expression in cultured retinal pericytes was TIE-2 gene encodes a protein of 1122 amino acids with its analyzed by using ELISA, Western Blot analysis, and flow cy- extracellular region containing three EGF-like repeats and tometry. CD13 (aminopeptidase N) expression in pericytes three repeats with fibronectin type III homology located after was determined by Western blot analysis and Ang effects ver- the second Ig loop. The intracellular portion of Tie-2 contains ified with Tie-2 antisense treatment. Cell proliferation was two tyrosine kinase domains. Tie-2-deficient mice die at around monitored by crystal violet uptake, and pericyte migration was embryonic day 10.5 and exhibit profound vascular defects asse