热痹康汤对胶原诱导性关节炎大鼠滑膜血管内皮生长因子影响.docVIP

热痹康汤对胶原诱导性关节炎大鼠滑膜血管内皮生长因子的影响 　　【摘 要】目的：观察清热解毒抗风湿方药――热痹康汤对胶原诱导性关节炎（CIA）大鼠滑膜血管内皮生长因子（VEGF）的影响。方法：将60只清洁级雌性Wistar大鼠随机分为空白对照组，模型对照组，甲氨蝶呤组，热痹康汤低、中、高剂量组，每组10只。除空白对照组外，其余各组大鼠采用弗氏不完全佐剂联合牛Ⅱ型胶原蛋白诱导CIA大鼠模型。造模成功后开始给药，模型对照组给予生理盐水灌胃，热痹康汤低、中、高剂量组分别给予热痹康汤提取物低、中、高剂量灌胃，甲氨蝶呤组给予甲氨蝶呤灌胃，空白对照组大鼠自由饮水和进食。观察不同剂量热痹康汤对CIA大鼠的关节肿胀指数及其滑膜VEGF的影响。结果：热痹康汤各剂量组及甲氨蝶呤组大鼠的关节肿胀指数均低于模型对照组（P 0.05），高剂量热痹康汤作用优于甲氨蝶呤（P 0.05）。结论：热痹康汤治疗类风湿关节炎的机制是通过抑制滑膜VEGF的产生而实现的 【关键词】 胶原诱导性关节炎；关节炎，类风湿；热痹康汤；血管内皮生长因子；大鼠 Effect of Rebikang Tang（热痹康汤）on Vascular Endothelial Growth Factor in Rats with Collagen-induced Arthritis PANG Xue-feng，LIU Sha-sha，LI Yu-ling，LIU Huan，MENG Yu-hua，YAO Ping，FENG Yu-qing，WU Yan-hong，TANG Li-ping，LEI Zong-shang，SHU Jian-long，WANG Si-chao，DU Yong-yi，LIN Ju 【ABSTRACT】Objective：To observe the effect of Rebikang Tang（热痹康汤―a prescription of clearing away heat and toxic materials and anti-rheumatism）on vascular endothelial growth factor（VEGF）in rats with collagen-induced arthritis（CIA）.Methods：Sixty clean-grade female Wistar rats were randomly divided into a blank control group，a model control group，a methotrexate group，and low，medium and high-dose Rebikang Tang groups，10 rats in each group.Except the blank control group，the rats in the other groups were induced by the incomplete adjuvant combined with collagen typeⅡto establish CIA rat models.The model control group was given intragastric administration of physiological saline，the low，medium and high-dose Rebikang Tang groups were given corresponding dose of Rebikang Tang，the methotrexate group was given intragastric administration of methotrexate and the rats in the control group could drink water and eat food freely.The effects of different doses of Rebikang on joint swelling index and VEGF were observed.Results：The joint swelling indexes in each dose of Rebikang group and the methotrexate group were lower than that of the model group（P 0.05） and the effect in the high-dose Rebikang group was superior to that of the methotrexate group（P