电刺激对富脂性胰岛素抵抗骨骼肌细胞糖代谢影响.docVIP

电刺激对富脂性胰岛素抵抗骨骼肌细胞糖代谢的影响 　　摘 要：棕榈酸孵育C2C12细胞建立富脂性胰岛素抵抗骨骼肌细胞模型，观察一定强度的电刺激引发收缩对其糖代谢的影响。选用不同梯度浓度的棕榈酸孵育分化5 d的C2C12细胞16 h，检测各组细胞培养液中葡萄糖剩余量和细胞活性，选取合适的棕榈酸浓度建立其诱导的胰岛素抵抗细胞模型。随后实验分为4组：对照组（C组）、电刺激组（EPS组）、棕榈酸组（PA组）、电刺激+棕榈酸组（EPS+PA组），以15 V、30 ms、2 Hz的强度电刺激60 min，收样测定细胞培养液葡萄糖剩余量、AMPK-α2 mRNA和GLUT4蛋白表达量。结果显示，（1）0.5 mmol/L棕榈酸溶液孵育16 h，与C组相比，LDH、MTT无显著变化（P0.05），葡萄糖剩余量明显增加（P0.05）；（2）与C组相比，PA组葡萄糖剩余量显著增加（P0.05）。结果说明：棕榈酸（0.5 mmol/L）孵育分化5 d的C2C12细胞16 h，可以导致其富脂性胰岛素抵抗的发生。15 V、30 ms、2 Hz的强度电刺激60 min，可以提高胰岛素抵抗骨骼肌细胞对葡萄糖的吸收，增加AMPK-α2mRNA和GLUT4蛋白的表达，有助于改善富脂性胰岛素抵抗骨骼肌细胞的糖代谢 关 键 词：运动生物化学；骨骼肌细胞；棕榈酸；胰岛素抵抗；电刺激；糖代谢；小鼠 中图分类号：G804.7 文献标志码：A 文章编号：1006-7116（2016）06-0129-06 Effects of electric stimulation on lipid rich insulin resisting the sugar metabolism of skeletal muscle cells PAN Hong-ying1，XU Xiao-yang1，JIA Zhang-zhi2 （1.School of Physical Education，South China Normal University，Guangzhou 510006，China； 2. Sports Science Research Institute of Guangdong Province，Guangzhou 510100，China） Abstract： By using palmitic acid to incubate C2C12 cells， the authors established a model of lipid rich insulin resisting skeletal muscle cells， so as to observe the effects of contraction triggered by a certain intensity of electric stimulation on the cells sugar metabolism. The authors used different gradient concentrations of palmitic acid to incubate 5d differentiated C2C12 cells for 16h， measured glucose remaining amount and cell activity in the cell culture fluid of various groups， selected an appropriate palmitic acid concentration to establish a model of insulin resisting the cells， which was induced by such a concentration， then divided the experiment into 4 groups： a control group （C）， an electric stimulation group （EPS）， a palmitic acid group （PA）， and an electric stimulation + palmitic acid group （EPS+PA ）， applied an intensity of （15 V， 30 ms， 2 Hz） of electric stimulation for 60min， collected the samples and measured glucose remaining amount， AMPK-α2 mRNA and GLUT4 protein expression le