蔬菜废弃物固体发酵生产饲料蛋白.pdfVIP

43 2007 4 ( ) Vol1 43 2007 No1 4 Journal of Northw est Normal U niversit y ( Natural Science) 85 蔬菜废弃物固体发酵生产饲料蛋白 张 继, 武光朋, 高义霞, 冯 涛, 高 超 ( , 730070) : 通过平板点种刺激圈及混合菌种发酵试验, 确定高山 菜废弃物发酵的最佳菌种组合及其最佳接种量和接 种比例; 通过固体发酵试验, 确定了发酵培养基的最佳氮源无机盐以及添加量; 采用正交实验, 确定了最佳发酵条 件. 结果表明: 采用绿色木霉+ 白地霉+ 产朊假丝酵母为发酵菌种, 加入 % 的尿素 1 5% 的硫酸铵01 2% 硫酸镁 01 4% 的磷酸氢二钾和01 4% 的过磷酸钙, 采用不灭菌固体发酵工艺, 并按接种 0% , 比例为 B2B2, 蔬菜废弃物和麸 皮( 比例为85B 5) , 在 L 的三角瓶中投放量 00 g, 温度 27 e 下发酵96 h, 产物蛋白质含量高达 51 97% , 提高了 75% , 营养成分平衡后, 易作为饲料. : 高山 菜; 固体发酵; 饲料蛋白 : S 38 : A : 00 -988( 2007) 04-0085-05 FeedingOprotein producing by solidOfermentation from vegetable w astes ZHANG Ji, WU GuangOpeng, GAO YiOxia, FENG Tao, GA O Chao ( College of Life Science, Northw est Normal University, Lanzhou 730070, Gansu, China) Abstract: T he suitable inoculation proportion and strain combination for fermentation of vegetable w aste are obtained by conventional screening methods and mixed strain combination experiment; the optimum nitrogen source, abioOsalt and their inoculation ratio of the culturing medium are obtained by solidOstate fermentation experiment; and the fermentation conditions are obtained by perpendicular orthogonal test. Results show that w hen the fermentation strain ratio of Trichoderma v iride, Geotrichum candidum and Candida utilis is B2B2, and the inoculation is about 0%, ( NH2) 2 CO %, ( NH4 ) 2 SO4 15%, MgSO4 012% , K2 H PO4 014% and Ca( H2 PO4 ) 2 #H2 O 014%, adopting a craft of the immortalization germ solidO ferment, throw ing 00 g fermentation germ in the triangle bottle w ith L, temperature 27 e , fermenting 96 h, the outcome protein content is up to 5197