鞘内注射#gamma#--氨基丁酸转运体抑制剂NO--711抑制坐骨神经慢性挤压伤大鼠神经病理性痛觉过敏教案.pdfVIP

Acta Physiologica Sinica , April 25, 2005, 57 (2): 233-239 233 Research Paper Inhibition of thermal hyperalgesia and tactile allodynia by intrathecal administration of γ-aminobutyric acid transporter-1 inhibitor NO-711 in rats with chronic constriction injury 1,3,* 2 1 2 2 2 ZHU Shan-Shan , ZENG Yin-Ming , WANG Jun-Ke , YAN Rong , NIE Xin , CAO Jun-Li 1 Department of Anesthesiology, the First Affiliated Hospital, China Medical University, Shenyang 110001, China; 2 Jiangsu Institute of Anesthesiology, Xuzhou Medical College, Xuzhou 221002, China; 3 Department of Anesthesiology, the 3rd Hospital of Xuzhou, Xuzhou 221002, China Abstract: The present study was undertaken to explore the role of γ-aminobutyric acid transporters in the neuropathic pain. On the chronic constriction injury (CCI) rats 4 doses (5, 10, 20, 40 µg in group N5, N10, N20, N40, respectively) of specific γ-aminobutyric acid transporter-1 inhibitor NO-711 or normal saline (in group NS) were intrathecally administered before sciatic nerve ligation (pre- treatment) or at the third day after ligation (post-treatment). The paw withdrawl latency (PWL) from a noxious thermal stimulus and paw withdrawl mechanical threshold (PWMT) of von Frey filament was used as measure of thermal hyperalgesia and tactile allodynia respectively. The results demonstrated that post-treatment of NO-711 significantly suppressed thermal hyperalgesia and allodynia in CCI rats (P0.05, P0.01), the inhibitory effect lasted for 2 h (N40 group) and 4 h (N20 group) respectively. NO-711 inhibited thermal hyperalgesia induced by CCI in a dose-dependent manner. Intrathecal pretreatment with different doses of NO-711 delayed the occurrence of thermal hyperalgesia, but could not delay the emergence of allodynia induced by CCI. This study indicates that γ- a