溶血磷脂酸对培养小鼠大脑皮层神经元的双重作用抗凋亡及促凋亡.pdf

ZHENG Zhao-Qing et al: Dual Action of LPA in Cultured Cortical Neurons: Survival and Apoptogenic Acta Physiologica Sinica, April 25, 2004, 56(2): 163-171 163 Research Paper Dual action of lysophosphatidic acid in cultured cortical neurons: survival and apoptogenic 1 2 1* ZHENG Zhao-Qing , FANG Xian-Jun , QIAO Jian-Tian 1Department of Neurobiology, Shanxi Medical University, Taiyuan, Shanxi 030001, China 2 M . D. Anderson Cancer Center, Department of Molecular Therapeutic, 1515 Holcombe Blvd., Houston, TX 77030, USA Abstract: The effect of lysophosphatidic acid (LPA), with a wide range of its different concentrations, upon cultured mouse cortical neurons was assessed by electrophoresis of DNA fragments, HO33342 and TUNEL stainings, and also by ultrastructural examination at times. The results showed that administration of LPA at lower concentrations (0.1~30 µmol/L) dose-dependently protected cortical neurons from apoptosis that was induced by deprivation of serum from the cultural medium, while 50 µmol/L or higher concentrations of LPA failed to show this effect; and moreover, the concentrations higher than 50 µmol/L induced apoptosis in neurons cultured in serum- containing complete medium. These results suggest that a moderate concentration of LPA may play as a survival factor in apoptotic cortical neurons, while an excessive level of LPA induces apoptosis in neurons cultured in complete medium. Key words: lysophosphatidic acid; apoptosis; survival factor; serum-free medium; complete medium; cortical neurons; mice 1 2 1,* 1 , 030001; 2 , 77030, : DNA HO33342 TUNEL