响应面法优化超声波辅助提取冬虫夏草多糖工艺研究.pdf

Modern Food 工程技术 doi:6736/j.cnki.cn41-1434/ts.2016.22.042 10.1 响应面法优化超声波辅助提取冬虫夏草 多糖工艺研究 Cordyceps sinensis by Response Surface Method 1 1 2 ◎ 刘忠宇 ，杜　静 ，王　瑜 （1. 贵州省贞丰县市场监督检测所，贵州　贞丰　562200； 2. 贵州省中国科学院天然产物化学重点实验室，贵州　贵阳　550002) 1 1 2 Liu Zhongyu , Du　Jing , Wang　Yu （1.Market Supervision and Inspection Institute of Zhenfeng County, Guizhou Province, Zhenfeng　562200, China; 2. The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang　550002, China） 摘　要：为采用响应面优化法对超声波辅助提取冬虫夏草多糖的工艺参数进行优化，设计单因素实验，确定 影响提取率的几个因素的大概范围，即超声波提取功率、提取温度、提取时间、提取料液比。然后设计响应面实 验对工艺参数进行优化，通过查阅文献，选定蒽酮 - 硫酸法测定冬虫夏草多糖含量，在 490 nm 的波长下测定吸光值， 算出多糖含量，以此作为响应面设计的响应值。结果表明，超声波辅助提取冬虫夏草多糖工艺参数为超声波功率 为 300 W，提取时间为 30 min，提取温度为 45 ℃，料液比为 1/50。在此参数下冬虫夏草多糖提取率最大。 关键词：冬虫夏草；响应面；超声波；多糖 Abstract： In order to use the response surface optimization method to optimize the process parameters for ultrasonic assisted extraction of polysaccharide of cordyceps sinensis, single factor experiments were designed to determine the range of factors afecting the extraction rate, including power ultrasonic extraction, extraction temperature, extraction time, extraction and liquid ratio. Then the response surface experiment was designed to optimize the process parameters, through the literature, the anthrone - sulfuric acid method was chose to determined polysaccharide content of Cordyceps sinensis, the absorbance value was measured at the wavelength of 49